Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula. / Azarakhsh, Mahboobeh; Rumyantsev, Andrey M.; Lebedeva, Maria A.; Lutova, Lyudmila A.
в: PLoS ONE, Том 15, № 4, e0232352, 30.04.2020.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula
AU - Azarakhsh, Mahboobeh
AU - Rumyantsev, Andrey M.
AU - Lebedeva, Maria A.
AU - Lutova, Lyudmila A.
N1 - Azarakhsh M, Rumyantsev AM, Lebedeva MA, Lutova LA (2020) Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula. PLoS ONE 15(4): e0232352. https://doi.org/10.1371/journal.pone.0232352
PY - 2020/4/30
Y1 - 2020/4/30
N2 - Cytokinin is an important regulator of symbiotic nodule development. Recently, KNOTTED1-LIKE HOMEOBOX 3 transcription factor (TF) was shown to regulate symbiotic nodule development possibly via the activation of cytokinin biosynthesis genes. However, the direct interaction between the KNOX3 TF and its target genes has not been investigated up to date. Here, using EMSA analysis and SPR-based assay, we found that MtKNOX3 homeodomain directly binds to the regulatory sequences of the MtLOG1, MtLOG2, and MtIPT3 genes involved in nodulation in Medicago truncatula. Moreover, we showed that MtLOG2 and MtIPT3 expression patterns partially overlap with MtKNOX3 expression in developing nodules as it was shown by promoter:GUS analysis. Our data suggest that MtKNOX3 TF may directly activate the MtLOG1, MtLOG2, and MtIPT3 genes during nodulation thereby increasing cytokinin biosynthesis in developing nodules.
AB - Cytokinin is an important regulator of symbiotic nodule development. Recently, KNOTTED1-LIKE HOMEOBOX 3 transcription factor (TF) was shown to regulate symbiotic nodule development possibly via the activation of cytokinin biosynthesis genes. However, the direct interaction between the KNOX3 TF and its target genes has not been investigated up to date. Here, using EMSA analysis and SPR-based assay, we found that MtKNOX3 homeodomain directly binds to the regulatory sequences of the MtLOG1, MtLOG2, and MtIPT3 genes involved in nodulation in Medicago truncatula. Moreover, we showed that MtLOG2 and MtIPT3 expression patterns partially overlap with MtKNOX3 expression in developing nodules as it was shown by promoter:GUS analysis. Our data suggest that MtKNOX3 TF may directly activate the MtLOG1, MtLOG2, and MtIPT3 genes during nodulation thereby increasing cytokinin biosynthesis in developing nodules.
KW - Cytokinins/biosynthesis
KW - Gene Expression Regulation, Developmental
KW - Gene Expression Regulation, Plant
KW - Homeodomain Proteins/genetics
KW - Medicago truncatula/genetics
KW - Plant Proteins/genetics
KW - Promoter Regions, Genetic
KW - Transcription Factors/genetics
KW - PLANT
KW - RNA
KW - KNOTTED CLASS
KW - HOMEODOMAIN PROTEIN
KW - RESPONSES
KW - ARABIDOPSIS
KW - MAP
UR - http://www.scopus.com/inward/record.url?scp=85084170125&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/c7a90d82-1292-30ae-ae94-9442112210e5/
U2 - 10.1371/journal.pone.0232352
DO - 10.1371/journal.pone.0232352
M3 - Article
C2 - 32353031
AN - SCOPUS:85084170125
VL - 15
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 4
M1 - e0232352
ER -
ID: 70929235