Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula

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Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula. / Azarakhsh, Mahboobeh ; Rumyantsev, Andrey M.; Lebedeva, Maria A.; Lutova, Lyudmila A.

In: PLoS ONE, Vol. 15, No. 4, e0232352, 30.04.2020.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{69fbc46b834f4a09877eaeb6fbf8a892,

title = "Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula",

abstract = "Cytokinin is an important regulator of symbiotic nodule development. Recently, KNOTTED1-LIKE HOMEOBOX 3 transcription factor (TF) was shown to regulate symbiotic nodule development possibly via the activation of cytokinin biosynthesis genes. However, the direct interaction between the KNOX3 TF and its target genes has not been investigated up to date. Here, using EMSA analysis and SPR-based assay, we found that MtKNOX3 homeodomain directly binds to the regulatory sequences of the MtLOG1, MtLOG2, and MtIPT3 genes involved in nodulation in Medicago truncatula. Moreover, we showed that MtLOG2 and MtIPT3 expression patterns partially overlap with MtKNOX3 expression in developing nodules as it was shown by promoter:GUS analysis. Our data suggest that MtKNOX3 TF may directly activate the MtLOG1, MtLOG2, and MtIPT3 genes during nodulation thereby increasing cytokinin biosynthesis in developing nodules.",

keywords = "Cytokinins/biosynthesis, Gene Expression Regulation, Developmental, Gene Expression Regulation, Plant, Homeodomain Proteins/genetics, Medicago truncatula/genetics, Plant Proteins/genetics, Promoter Regions, Genetic, Transcription Factors/genetics, PLANT, RNA, KNOTTED CLASS, HOMEODOMAIN PROTEIN, RESPONSES, ARABIDOPSIS, MAP",

author = "Mahboobeh Azarakhsh and Rumyantsev, {Andrey M.} and Lebedeva, {Maria A.} and Lutova, {Lyudmila A.}",

note = " Azarakhsh M, Rumyantsev AM, Lebedeva MA, Lutova LA (2020) Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula. PLoS ONE 15(4): e0232352. https://doi.org/10.1371/journal.pone.0232352",

year = "2020",

month = apr,

day = "30",

doi = "10.1371/journal.pone.0232352",

language = "English",

volume = "15",

journal = "PLoS ONE",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "4",

}

RIS

TY - JOUR

T1 - Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula

AU - Azarakhsh, Mahboobeh

AU - Rumyantsev, Andrey M.

AU - Lebedeva, Maria A.

AU - Lutova, Lyudmila A.

N1 - Azarakhsh M, Rumyantsev AM, Lebedeva MA, Lutova LA (2020) Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula. PLoS ONE 15(4): e0232352. https://doi.org/10.1371/journal.pone.0232352

PY - 2020/4/30

Y1 - 2020/4/30

N2 - Cytokinin is an important regulator of symbiotic nodule development. Recently, KNOTTED1-LIKE HOMEOBOX 3 transcription factor (TF) was shown to regulate symbiotic nodule development possibly via the activation of cytokinin biosynthesis genes. However, the direct interaction between the KNOX3 TF and its target genes has not been investigated up to date. Here, using EMSA analysis and SPR-based assay, we found that MtKNOX3 homeodomain directly binds to the regulatory sequences of the MtLOG1, MtLOG2, and MtIPT3 genes involved in nodulation in Medicago truncatula. Moreover, we showed that MtLOG2 and MtIPT3 expression patterns partially overlap with MtKNOX3 expression in developing nodules as it was shown by promoter:GUS analysis. Our data suggest that MtKNOX3 TF may directly activate the MtLOG1, MtLOG2, and MtIPT3 genes during nodulation thereby increasing cytokinin biosynthesis in developing nodules.

AB - Cytokinin is an important regulator of symbiotic nodule development. Recently, KNOTTED1-LIKE HOMEOBOX 3 transcription factor (TF) was shown to regulate symbiotic nodule development possibly via the activation of cytokinin biosynthesis genes. However, the direct interaction between the KNOX3 TF and its target genes has not been investigated up to date. Here, using EMSA analysis and SPR-based assay, we found that MtKNOX3 homeodomain directly binds to the regulatory sequences of the MtLOG1, MtLOG2, and MtIPT3 genes involved in nodulation in Medicago truncatula. Moreover, we showed that MtLOG2 and MtIPT3 expression patterns partially overlap with MtKNOX3 expression in developing nodules as it was shown by promoter:GUS analysis. Our data suggest that MtKNOX3 TF may directly activate the MtLOG1, MtLOG2, and MtIPT3 genes during nodulation thereby increasing cytokinin biosynthesis in developing nodules.

KW - Cytokinins/biosynthesis

KW - Gene Expression Regulation, Developmental

KW - Gene Expression Regulation, Plant

KW - Homeodomain Proteins/genetics

KW - Medicago truncatula/genetics

KW - Plant Proteins/genetics

KW - Promoter Regions, Genetic

KW - Transcription Factors/genetics

KW - PLANT

KW - RNA

KW - KNOTTED CLASS

KW - HOMEODOMAIN PROTEIN

KW - RESPONSES

KW - ARABIDOPSIS

KW - MAP

UR - http://www.scopus.com/inward/record.url?scp=85084170125&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/c7a90d82-1292-30ae-ae94-9442112210e5/

U2 - 10.1371/journal.pone.0232352

DO - 10.1371/journal.pone.0232352

M3 - Article

C2 - 32353031

AN - SCOPUS:85084170125

VL - 15

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 4

M1 - e0232352

ER -

ID: 70929235