TY - JOUR

T1 - Biological activity of analogs of the peptide hormone luliberin in the regulation of the immune response of T cells

AU - Kazakova, T. B.

AU - Burov, S. V.

AU - Grishina, T. V.

AU - Myul'berg, A. A.

AU - Korneva, E. A.

AU - Novikova, N.S.

AU - Semko, T.V.

AU - Golovko, O.I.

PY - 1996/9/1

Y1 - 1996/9/1

N2 - The ability of the antitumor analogs of luliberin (LH-RH), a hypothalamic peptide hormone, to stimulate the immune function of T cells is examined in experiments with the gene coding for interleukin-2 (IL-2). Recombinant MIL2C or 4xPu DNA containing the marker gene of chloramphenicol acetyltransferase (CAT) under the control of a 2.2 kb promoter of murine IL-2 gene or four copies of purine-rich element (from -292 to -246 base pairs), respectively, is injected in Xenopus laevis oocytes. The promoter activity is blocked by inoculation of the protein fraction of nuclear extracts from resting mouse splenic T cells. The IL-2 gene promoter is dereppressed after injection of the short LH-RH analog L1 (7 amino acid residues) into the oocyte nucleus or cytoplasm. The addition of L1 or L2 (an LH-RH analog consisting of 10 amino acid residues) to the incubation medium activates mouse splenic T cells and stimulates the synthesis of IL-2 mRNA 2-to 3-fold more intensely than ConA+rIL-2, judging from dot-blot and in situ hybridization data. Cytological analysis of cell culture shows that the presence of L1 and L2 peptides in the culture medium promotes differentiation of T cells. It is hypothesized that the antitumor activity of these peptides is associated with the stimulation of IL-2 synthesis.

AB - The ability of the antitumor analogs of luliberin (LH-RH), a hypothalamic peptide hormone, to stimulate the immune function of T cells is examined in experiments with the gene coding for interleukin-2 (IL-2). Recombinant MIL2C or 4xPu DNA containing the marker gene of chloramphenicol acetyltransferase (CAT) under the control of a 2.2 kb promoter of murine IL-2 gene or four copies of purine-rich element (from -292 to -246 base pairs), respectively, is injected in Xenopus laevis oocytes. The promoter activity is blocked by inoculation of the protein fraction of nuclear extracts from resting mouse splenic T cells. The IL-2 gene promoter is dereppressed after injection of the short LH-RH analog L1 (7 amino acid residues) into the oocyte nucleus or cytoplasm. The addition of L1 or L2 (an LH-RH analog consisting of 10 amino acid residues) to the incubation medium activates mouse splenic T cells and stimulates the synthesis of IL-2 mRNA 2-to 3-fold more intensely than ConA+rIL-2, judging from dot-blot and in situ hybridization data. Cytological analysis of cell culture shows that the presence of L1 and L2 peptides in the culture medium promotes differentiation of T cells. It is hypothesized that the antitumor activity of these peptides is associated with the stimulation of IL-2 synthesis.

KW - Interleukin-2 gene

KW - Luliberin

KW - mRNA synthesis

KW - люлиберин, ген интерлейкина-2, синтез мРНК

UR - http://www.scopus.com/inward/record.url?scp=26944452854&partnerID=8YFLogxK

M3 - Article

C2 - 8974494

AN - SCOPUS:0030226878

VL - 122

SP - 943

EP - 946

JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

SN - 0007-4888

IS - 9

ER -