Research output: Contribution to journal › Article › peer-review
Biological activity of analogs of the peptide hormone luliberin in the regulation of the immune response of T cells. / Kazakova, T. B.; Burov, S. V.; Grishina, T. V.; Myul'berg, A. A.; Korneva, E. A.; Novikova, N.S.; Semko, T.V.; Golovko, O.I.
In: Bulletin of Experimental Biology and Medicine, Vol. 122, No. 9, 01.09.1996, p. 943-946.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Biological activity of analogs of the peptide hormone luliberin in the regulation of the immune response of T cells
AU - Kazakova, T. B.
AU - Burov, S. V.
AU - Grishina, T. V.
AU - Myul'berg, A. A.
AU - Korneva, E. A.
AU - Novikova, N.S.
AU - Semko, T.V.
AU - Golovko, O.I.
PY - 1996/9/1
Y1 - 1996/9/1
N2 - The ability of the antitumor analogs of luliberin (LH-RH), a hypothalamic peptide hormone, to stimulate the immune function of T cells is examined in experiments with the gene coding for interleukin-2 (IL-2). Recombinant MIL2C or 4xPu DNA containing the marker gene of chloramphenicol acetyltransferase (CAT) under the control of a 2.2 kb promoter of murine IL-2 gene or four copies of purine-rich element (from -292 to -246 base pairs), respectively, is injected in Xenopus laevis oocytes. The promoter activity is blocked by inoculation of the protein fraction of nuclear extracts from resting mouse splenic T cells. The IL-2 gene promoter is dereppressed after injection of the short LH-RH analog L1 (7 amino acid residues) into the oocyte nucleus or cytoplasm. The addition of L1 or L2 (an LH-RH analog consisting of 10 amino acid residues) to the incubation medium activates mouse splenic T cells and stimulates the synthesis of IL-2 mRNA 2-to 3-fold more intensely than ConA+rIL-2, judging from dot-blot and in situ hybridization data. Cytological analysis of cell culture shows that the presence of L1 and L2 peptides in the culture medium promotes differentiation of T cells. It is hypothesized that the antitumor activity of these peptides is associated with the stimulation of IL-2 synthesis.
AB - The ability of the antitumor analogs of luliberin (LH-RH), a hypothalamic peptide hormone, to stimulate the immune function of T cells is examined in experiments with the gene coding for interleukin-2 (IL-2). Recombinant MIL2C or 4xPu DNA containing the marker gene of chloramphenicol acetyltransferase (CAT) under the control of a 2.2 kb promoter of murine IL-2 gene or four copies of purine-rich element (from -292 to -246 base pairs), respectively, is injected in Xenopus laevis oocytes. The promoter activity is blocked by inoculation of the protein fraction of nuclear extracts from resting mouse splenic T cells. The IL-2 gene promoter is dereppressed after injection of the short LH-RH analog L1 (7 amino acid residues) into the oocyte nucleus or cytoplasm. The addition of L1 or L2 (an LH-RH analog consisting of 10 amino acid residues) to the incubation medium activates mouse splenic T cells and stimulates the synthesis of IL-2 mRNA 2-to 3-fold more intensely than ConA+rIL-2, judging from dot-blot and in situ hybridization data. Cytological analysis of cell culture shows that the presence of L1 and L2 peptides in the culture medium promotes differentiation of T cells. It is hypothesized that the antitumor activity of these peptides is associated with the stimulation of IL-2 synthesis.
KW - Interleukin-2 gene
KW - Luliberin
KW - mRNA synthesis
KW - люлиберин, ген интерлейкина-2, синтез мРНК
UR - http://www.scopus.com/inward/record.url?scp=26944452854&partnerID=8YFLogxK
M3 - Article
C2 - 8974494
AN - SCOPUS:0030226878
VL - 122
SP - 943
EP - 946
JO - Bulletin of Experimental Biology and Medicine
JF - Bulletin of Experimental Biology and Medicine
SN - 0007-4888
IS - 9
ER -
ID: 41855264