Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Amphiphysin is a component of clathrin coats formed during synaptic vesicle recycling at the lamprey giant synapse. / Evergren, Emma; Marcucci, Melissa; Tomilin, Nikolay; Löw, Peter; Slepnev, Vladimir; Andersson, Fredrik; Gad, Helge; Brodin, Lennart; De Camilli, Pietro; Shupliakov, Oleg.
в: Traffic, Том 5, № 7, 01.07.2004, стр. 514-528.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Amphiphysin is a component of clathrin coats formed during synaptic vesicle recycling at the lamprey giant synapse
AU - Evergren, Emma
AU - Marcucci, Melissa
AU - Tomilin, Nikolay
AU - Löw, Peter
AU - Slepnev, Vladimir
AU - Andersson, Fredrik
AU - Gad, Helge
AU - Brodin, Lennart
AU - De Camilli, Pietro
AU - Shupliakov, Oleg
PY - 2004/7/1
Y1 - 2004/7/1
N2 - Amphiphysin is a protein enriched at mammalian synapses thought to function as a clathrin accessory factor in synaptic vesicle endocytosis. Here we examine the involvement of amphiphysin in synaptic vesicle recycling at the giant synapse in the lamprey. We show that amphiphysin resides in the synaptic vesicle cluster at rest and relocates to sites of endocytosis during synaptic activity. It accumulates at coated pits where its SH3 domain, but not its central clathrin/AP-2-binding (CLAP) region, is accessible for antibody binding. Microinjection of antibodies specifically directed against the CLAP region inhibited recycling of synaptic vesicles and caused accumulation of clathrin-coated intermediates with distorted morphology, including flat patches of coated presynaptic membrane. Our data provide evidence for an activity-dependent redistribution of amphiphysin in intact nerve terminals and show that amphiphysin is a component of presynaptic clathrin-coated intermediates formed during synaptic vesicle recycling.
AB - Amphiphysin is a protein enriched at mammalian synapses thought to function as a clathrin accessory factor in synaptic vesicle endocytosis. Here we examine the involvement of amphiphysin in synaptic vesicle recycling at the giant synapse in the lamprey. We show that amphiphysin resides in the synaptic vesicle cluster at rest and relocates to sites of endocytosis during synaptic activity. It accumulates at coated pits where its SH3 domain, but not its central clathrin/AP-2-binding (CLAP) region, is accessible for antibody binding. Microinjection of antibodies specifically directed against the CLAP region inhibited recycling of synaptic vesicles and caused accumulation of clathrin-coated intermediates with distorted morphology, including flat patches of coated presynaptic membrane. Our data provide evidence for an activity-dependent redistribution of amphiphysin in intact nerve terminals and show that amphiphysin is a component of presynaptic clathrin-coated intermediates formed during synaptic vesicle recycling.
KW - Amphiphysin
KW - Antibody microinjection
KW - Clathrin-mediated endocytosis
KW - Electron microscopy
KW - Immunocytochemistry
KW - Synaptic vesicle trafficking
UR - http://www.scopus.com/inward/record.url?scp=3142757111&partnerID=8YFLogxK
U2 - 10.1111/j.1398-9219.2004.00198.x
DO - 10.1111/j.1398-9219.2004.00198.x
M3 - Article
C2 - 15180828
AN - SCOPUS:3142757111
VL - 5
SP - 514
EP - 528
JO - Traffic
JF - Traffic
SN - 1398-9219
IS - 7
ER -
ID: 40832744