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Amphiphysin is a component of clathrin coats formed during synaptic vesicle recycling at the lamprey giant synapse. / Evergren, Emma; Marcucci, Melissa; Tomilin, Nikolay; Löw, Peter; Slepnev, Vladimir; Andersson, Fredrik; Gad, Helge; Brodin, Lennart; De Camilli, Pietro; Shupliakov, Oleg.

в: Traffic, Том 5, № 7, 01.07.2004, стр. 514-528.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Evergren, E, Marcucci, M, Tomilin, N, Löw, P, Slepnev, V, Andersson, F, Gad, H, Brodin, L, De Camilli, P & Shupliakov, O 2004, 'Amphiphysin is a component of clathrin coats formed during synaptic vesicle recycling at the lamprey giant synapse', Traffic, Том. 5, № 7, стр. 514-528. https://doi.org/10.1111/j.1398-9219.2004.00198.x

APA

Evergren, E., Marcucci, M., Tomilin, N., Löw, P., Slepnev, V., Andersson, F., Gad, H., Brodin, L., De Camilli, P., & Shupliakov, O. (2004). Amphiphysin is a component of clathrin coats formed during synaptic vesicle recycling at the lamprey giant synapse. Traffic, 5(7), 514-528. https://doi.org/10.1111/j.1398-9219.2004.00198.x

Vancouver

Evergren E, Marcucci M, Tomilin N, Löw P, Slepnev V, Andersson F и пр. Amphiphysin is a component of clathrin coats formed during synaptic vesicle recycling at the lamprey giant synapse. Traffic. 2004 Июль 1;5(7):514-528. https://doi.org/10.1111/j.1398-9219.2004.00198.x

Author

Evergren, Emma ; Marcucci, Melissa ; Tomilin, Nikolay ; Löw, Peter ; Slepnev, Vladimir ; Andersson, Fredrik ; Gad, Helge ; Brodin, Lennart ; De Camilli, Pietro ; Shupliakov, Oleg. / Amphiphysin is a component of clathrin coats formed during synaptic vesicle recycling at the lamprey giant synapse. в: Traffic. 2004 ; Том 5, № 7. стр. 514-528.

BibTeX

@article{e4b4f5f00b8a471eac159f3cfe96b147,
title = "Amphiphysin is a component of clathrin coats formed during synaptic vesicle recycling at the lamprey giant synapse",
abstract = "Amphiphysin is a protein enriched at mammalian synapses thought to function as a clathrin accessory factor in synaptic vesicle endocytosis. Here we examine the involvement of amphiphysin in synaptic vesicle recycling at the giant synapse in the lamprey. We show that amphiphysin resides in the synaptic vesicle cluster at rest and relocates to sites of endocytosis during synaptic activity. It accumulates at coated pits where its SH3 domain, but not its central clathrin/AP-2-binding (CLAP) region, is accessible for antibody binding. Microinjection of antibodies specifically directed against the CLAP region inhibited recycling of synaptic vesicles and caused accumulation of clathrin-coated intermediates with distorted morphology, including flat patches of coated presynaptic membrane. Our data provide evidence for an activity-dependent redistribution of amphiphysin in intact nerve terminals and show that amphiphysin is a component of presynaptic clathrin-coated intermediates formed during synaptic vesicle recycling.",
keywords = "Amphiphysin, Antibody microinjection, Clathrin-mediated endocytosis, Electron microscopy, Immunocytochemistry, Synaptic vesicle trafficking",
author = "Emma Evergren and Melissa Marcucci and Nikolay Tomilin and Peter L{\"o}w and Vladimir Slepnev and Fredrik Andersson and Helge Gad and Lennart Brodin and {De Camilli}, Pietro and Oleg Shupliakov",
year = "2004",
month = jul,
day = "1",
doi = "10.1111/j.1398-9219.2004.00198.x",
language = "English",
volume = "5",
pages = "514--528",
journal = "Traffic",
issn = "1398-9219",
publisher = "Wiley-Blackwell",
number = "7",

}

RIS

TY - JOUR

T1 - Amphiphysin is a component of clathrin coats formed during synaptic vesicle recycling at the lamprey giant synapse

AU - Evergren, Emma

AU - Marcucci, Melissa

AU - Tomilin, Nikolay

AU - Löw, Peter

AU - Slepnev, Vladimir

AU - Andersson, Fredrik

AU - Gad, Helge

AU - Brodin, Lennart

AU - De Camilli, Pietro

AU - Shupliakov, Oleg

PY - 2004/7/1

Y1 - 2004/7/1

N2 - Amphiphysin is a protein enriched at mammalian synapses thought to function as a clathrin accessory factor in synaptic vesicle endocytosis. Here we examine the involvement of amphiphysin in synaptic vesicle recycling at the giant synapse in the lamprey. We show that amphiphysin resides in the synaptic vesicle cluster at rest and relocates to sites of endocytosis during synaptic activity. It accumulates at coated pits where its SH3 domain, but not its central clathrin/AP-2-binding (CLAP) region, is accessible for antibody binding. Microinjection of antibodies specifically directed against the CLAP region inhibited recycling of synaptic vesicles and caused accumulation of clathrin-coated intermediates with distorted morphology, including flat patches of coated presynaptic membrane. Our data provide evidence for an activity-dependent redistribution of amphiphysin in intact nerve terminals and show that amphiphysin is a component of presynaptic clathrin-coated intermediates formed during synaptic vesicle recycling.

AB - Amphiphysin is a protein enriched at mammalian synapses thought to function as a clathrin accessory factor in synaptic vesicle endocytosis. Here we examine the involvement of amphiphysin in synaptic vesicle recycling at the giant synapse in the lamprey. We show that amphiphysin resides in the synaptic vesicle cluster at rest and relocates to sites of endocytosis during synaptic activity. It accumulates at coated pits where its SH3 domain, but not its central clathrin/AP-2-binding (CLAP) region, is accessible for antibody binding. Microinjection of antibodies specifically directed against the CLAP region inhibited recycling of synaptic vesicles and caused accumulation of clathrin-coated intermediates with distorted morphology, including flat patches of coated presynaptic membrane. Our data provide evidence for an activity-dependent redistribution of amphiphysin in intact nerve terminals and show that amphiphysin is a component of presynaptic clathrin-coated intermediates formed during synaptic vesicle recycling.

KW - Amphiphysin

KW - Antibody microinjection

KW - Clathrin-mediated endocytosis

KW - Electron microscopy

KW - Immunocytochemistry

KW - Synaptic vesicle trafficking

UR - http://www.scopus.com/inward/record.url?scp=3142757111&partnerID=8YFLogxK

U2 - 10.1111/j.1398-9219.2004.00198.x

DO - 10.1111/j.1398-9219.2004.00198.x

M3 - Article

C2 - 15180828

AN - SCOPUS:3142757111

VL - 5

SP - 514

EP - 528

JO - Traffic

JF - Traffic

SN - 1398-9219

IS - 7

ER -

ID: 40832744