Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Allotransplantation of pancreatic islet cells and antinuclear antibodies. / Galibin, O. V.; Sherstnov, M. I.; Emanuél', V. L.; Kashchenko, V. A.; Kofman, A. V.
в: Klinicheskaia laboratornaia diagnostika, № 3, 01.05.1996, стр. 27-29.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Allotransplantation of pancreatic islet cells and antinuclear antibodies
AU - Galibin, O. V.
AU - Sherstnov, M. I.
AU - Emanuél', V. L.
AU - Kashchenko, V. A.
AU - Kofman, A. V.
PY - 1996/5/1
Y1 - 1996/5/1
N2 - Antinuclear antibodies were detected and studied by indirect immunofluorescence in the sera of patients with insulin-dependent diabetes mellitus (IDDM) subjected to allotransplantation of pancreatic islet cell culture (PICT). Continuous cell lines L-41 (derivative of J-96) and HEp-2, a sensitive substrate for the detection of antinuclear antibodies, were used after enzyme treatment. Antinuclear antibodies were detected in 19.0% of diabetics in comparison with 1.5% in healthy volunteers.PICT evidently did not influence these antibodies, although a rise of hemagglutinins against sheep red cells after PICT was previously observed. The data indicate that the nuclear antigens of HEp-2 and L-41 against which antinuclear antibodies are directed are different from those found in human fetal pancreatic islet cells which have been implanted. A possible NAg maturity deficiency in fetal tissue may be another cause of their failure to affect the antinuclear antibodies.
AB - Antinuclear antibodies were detected and studied by indirect immunofluorescence in the sera of patients with insulin-dependent diabetes mellitus (IDDM) subjected to allotransplantation of pancreatic islet cell culture (PICT). Continuous cell lines L-41 (derivative of J-96) and HEp-2, a sensitive substrate for the detection of antinuclear antibodies, were used after enzyme treatment. Antinuclear antibodies were detected in 19.0% of diabetics in comparison with 1.5% in healthy volunteers.PICT evidently did not influence these antibodies, although a rise of hemagglutinins against sheep red cells after PICT was previously observed. The data indicate that the nuclear antigens of HEp-2 and L-41 against which antinuclear antibodies are directed are different from those found in human fetal pancreatic islet cells which have been implanted. A possible NAg maturity deficiency in fetal tissue may be another cause of their failure to affect the antinuclear antibodies.
UR - http://www.scopus.com/inward/record.url?scp=0030140444&partnerID=8YFLogxK
M3 - Article
C2 - 8925208
AN - SCOPUS:0030140444
SP - 27
EP - 29
JO - Клиническая лабораторная диагностика
JF - Клиническая лабораторная диагностика
SN - 0869-2084
IS - 3
ER -
ID: 119469113