Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Agrobacterial tumors interfere with nodulation and demonstrate the expression of nodulation-induced CLE genes in pea. / Samorodova, A P; Tvorogova, V E; Tkachenko, A A; Potsenkovskaya, E A; Lebedeva, М А; Tikhonovich, I A; Lutova, L А.
в: Journal of Plant Physiology, Том 221, 02.2018, стр. 94-100.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Agrobacterial tumors interfere with nodulation and demonstrate the expression of nodulation-induced CLE genes in pea
AU - Samorodova, A P
AU - Tvorogova, V E
AU - Tkachenko, A A
AU - Potsenkovskaya, E A
AU - Lebedeva, М А
AU - Tikhonovich, I A
AU - Lutova, L А
N1 - Copyright © 2017 Elsevier GmbH. All rights reserved.
PY - 2018/2
Y1 - 2018/2
N2 - CLAVATA (CLV) system including CLV1-like kinase and CLE-peptides is the part of the AON (autoregulation of nodulation) that controls nodule number in legume plants. Moreover, CLV system plays a key role in meristems, where it regulates the expression of WOX genes in organizing centers. Recently, we found that WOX5 homolog in pea is also expressed in nodules and in tumors induced by Agrobacterium tumefaciens. Based on this, we hypothesized that both nodules and agrobacterial tumors may be regulated by and may trigger the same components of AON, including the same WOX and CLV genes. Here, we found that pea plants with agrobacterial tumors induced prior to rhizobial inoculation had reduced the number of nodules. This effect was absent in pea sym29 mutant defective in CLV1-like kinase, the key component of AON. That suggests that agrobacterial tumors may produce a signal activating CLV1-like kinase and thereby decrease the nodule number. Since CLE peptides are known to act upstream of CLV1-like kinase, expression analysis of CLE genes has been performed both in developing nodules and tumors. Overall, 45 CLE genes were identified, and among them nine nodulation-induced CLEs were found in pea. In agrobacterial tumors, no expression of nodule-specific CLE genes the homologues of which inhibit nodulation in other legumes was observed. However, increased expression of two other nodulation-induced CLE genes was observed in agrobacterial tumors, suggesting that CLE genes are expressed in tumors that may still contribute to autoregulatory processes suppressing nodulation.
AB - CLAVATA (CLV) system including CLV1-like kinase and CLE-peptides is the part of the AON (autoregulation of nodulation) that controls nodule number in legume plants. Moreover, CLV system plays a key role in meristems, where it regulates the expression of WOX genes in organizing centers. Recently, we found that WOX5 homolog in pea is also expressed in nodules and in tumors induced by Agrobacterium tumefaciens. Based on this, we hypothesized that both nodules and agrobacterial tumors may be regulated by and may trigger the same components of AON, including the same WOX and CLV genes. Here, we found that pea plants with agrobacterial tumors induced prior to rhizobial inoculation had reduced the number of nodules. This effect was absent in pea sym29 mutant defective in CLV1-like kinase, the key component of AON. That suggests that agrobacterial tumors may produce a signal activating CLV1-like kinase and thereby decrease the nodule number. Since CLE peptides are known to act upstream of CLV1-like kinase, expression analysis of CLE genes has been performed both in developing nodules and tumors. Overall, 45 CLE genes were identified, and among them nine nodulation-induced CLEs were found in pea. In agrobacterial tumors, no expression of nodule-specific CLE genes the homologues of which inhibit nodulation in other legumes was observed. However, increased expression of two other nodulation-induced CLE genes was observed in agrobacterial tumors, suggesting that CLE genes are expressed in tumors that may still contribute to autoregulatory processes suppressing nodulation.
KW - Agrobacterial tumors
KW - Autoregulation of nodulation
KW - CLE
KW - CLV1-like kinase
KW - Nodule development
KW - WOX-CLV
KW - MUTANT
KW - CROWN-GALL
KW - NITRATE
KW - ROOT NODULATION
KW - PEPTIDES CONTROL
KW - AUTOREGULATION
KW - RECEPTOR KINASE
KW - LOTUS-JAPONICUS
KW - MERISTEM
KW - ARABIDOPSIS
UR - http://www.scopus.com/inward/record.url?scp=85038263590&partnerID=8YFLogxK
UR - http://www.mendeley.com/research/agrobacterial-tumors-interfere-nodulation-demonstrate-expression-nodulationinduced-cle-genes-pea
U2 - 10.1016/j.jplph.2017.12.005
DO - 10.1016/j.jplph.2017.12.005
M3 - Article
C2 - 29268087
VL - 221
SP - 94
EP - 100
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
SN - 0176-1617
ER -
ID: 18589756