TY - JOUR

T1 - A modern approach to demineralization of spicules in glass sponges (Porifera Hexactinellida) for the purpose of extraction and examination of the protein matrix

AU - Ehrlich, Herman

AU - Ereskovskii, A. V.

AU - Drozdov, A. L.

AU - Krylova, D. D.

AU - Hanke, T.

AU - Meissner, H.

AU - Heinemann, S.

AU - Worch, H.

N1 - Funding Information: The project was partially supported by a joint Russian–German program “DAAD–Mikhail Lomonosov” and the Foundation for Federal Support of Scientific Schools of the Russian Federation (grant NSH 1219.2003.4).

PY - 2006/5

Y1 - 2006/5

N2 - Glass sponges of the class Hexactinellida are a group of the most ancient multicellular animals, whose fossil remnants from the early Proterozoic have been registered. In order to demineralize the skeletal structures of the glass sponge Hyalonema sieboldi, we have used for the first time a strategy of slow leaching of the silicon-bearing component, based on the usage of alkaline solutions of sodium hydroxide, sodium dodecyl sulfate, and an anionic biosurfactant of a rhamnolipid nature. The obtained data unequivocally corroborate the presence of a fibrillar protein matrix functioning as a basis for silicon biomineralization in the basal spicules of H. sieboldi. Also, it has been found for the first time that the protein matrix is constructed of a collagenous protein. The technical approach proposed here might appear important for the study of the structural organization of skeletons in other silicon-bearing animals and, in an applied aspect, to work out new biomaterials for implantology and biocomposites, in order to use the latter as bioactive additives.

AB - Glass sponges of the class Hexactinellida are a group of the most ancient multicellular animals, whose fossil remnants from the early Proterozoic have been registered. In order to demineralize the skeletal structures of the glass sponge Hyalonema sieboldi, we have used for the first time a strategy of slow leaching of the silicon-bearing component, based on the usage of alkaline solutions of sodium hydroxide, sodium dodecyl sulfate, and an anionic biosurfactant of a rhamnolipid nature. The obtained data unequivocally corroborate the presence of a fibrillar protein matrix functioning as a basis for silicon biomineralization in the basal spicules of H. sieboldi. Also, it has been found for the first time that the protein matrix is constructed of a collagenous protein. The technical approach proposed here might appear important for the study of the structural organization of skeletons in other silicon-bearing animals and, in an applied aspect, to work out new biomaterials for implantology and biocomposites, in order to use the latter as bioactive additives.

KW - Biomineralization

KW - Biosilicates

KW - Collagen

KW - Glass sponges

KW - Hexactinellida

KW - Porifera

KW - Spicules

UR - http://www.scopus.com/inward/record.url?scp=33745950265&partnerID=8YFLogxK

U2 - 10.1134/S1063074006030060

DO - 10.1134/S1063074006030060

M3 - Article

AN - SCOPUS:33745950265

VL - 32

SP - 186

EP - 193

JO - Russian Journal of Marine Biology

JF - Russian Journal of Marine Biology

SN - 1063-0740

IS - 3

ER -