Research output: Contribution to journal › Article › peer-review
A modern approach to demineralization of spicules in glass sponges (Porifera Hexactinellida) for the purpose of extraction and examination of the protein matrix. / Ehrlich, Herman; Ereskovskii, A. V.; Drozdov, A. L.; Krylova, D. D.; Hanke, T.; Meissner, H.; Heinemann, S.; Worch, H.
In: Russian Journal of Marine Biology, Vol. 32, No. 3, 05.2006, p. 186-193.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - A modern approach to demineralization of spicules in glass sponges (Porifera Hexactinellida) for the purpose of extraction and examination of the protein matrix
AU - Ehrlich, Herman
AU - Ereskovskii, A. V.
AU - Drozdov, A. L.
AU - Krylova, D. D.
AU - Hanke, T.
AU - Meissner, H.
AU - Heinemann, S.
AU - Worch, H.
N1 - Funding Information: The project was partially supported by a joint Russian–German program “DAAD–Mikhail Lomonosov” and the Foundation for Federal Support of Scientific Schools of the Russian Federation (grant NSH 1219.2003.4).
PY - 2006/5
Y1 - 2006/5
N2 - Glass sponges of the class Hexactinellida are a group of the most ancient multicellular animals, whose fossil remnants from the early Proterozoic have been registered. In order to demineralize the skeletal structures of the glass sponge Hyalonema sieboldi, we have used for the first time a strategy of slow leaching of the silicon-bearing component, based on the usage of alkaline solutions of sodium hydroxide, sodium dodecyl sulfate, and an anionic biosurfactant of a rhamnolipid nature. The obtained data unequivocally corroborate the presence of a fibrillar protein matrix functioning as a basis for silicon biomineralization in the basal spicules of H. sieboldi. Also, it has been found for the first time that the protein matrix is constructed of a collagenous protein. The technical approach proposed here might appear important for the study of the structural organization of skeletons in other silicon-bearing animals and, in an applied aspect, to work out new biomaterials for implantology and biocomposites, in order to use the latter as bioactive additives.
AB - Glass sponges of the class Hexactinellida are a group of the most ancient multicellular animals, whose fossil remnants from the early Proterozoic have been registered. In order to demineralize the skeletal structures of the glass sponge Hyalonema sieboldi, we have used for the first time a strategy of slow leaching of the silicon-bearing component, based on the usage of alkaline solutions of sodium hydroxide, sodium dodecyl sulfate, and an anionic biosurfactant of a rhamnolipid nature. The obtained data unequivocally corroborate the presence of a fibrillar protein matrix functioning as a basis for silicon biomineralization in the basal spicules of H. sieboldi. Also, it has been found for the first time that the protein matrix is constructed of a collagenous protein. The technical approach proposed here might appear important for the study of the structural organization of skeletons in other silicon-bearing animals and, in an applied aspect, to work out new biomaterials for implantology and biocomposites, in order to use the latter as bioactive additives.
KW - Biomineralization
KW - Biosilicates
KW - Collagen
KW - Glass sponges
KW - Hexactinellida
KW - Porifera
KW - Spicules
UR - http://www.scopus.com/inward/record.url?scp=33745950265&partnerID=8YFLogxK
U2 - 10.1134/S1063074006030060
DO - 10.1134/S1063074006030060
M3 - Article
AN - SCOPUS:33745950265
VL - 32
SP - 186
EP - 193
JO - Russian Journal of Marine Biology
JF - Russian Journal of Marine Biology
SN - 1063-0740
IS - 3
ER -
ID: 90522626