TY - JOUR

T1 - A genomic blueprint of flax fungal parasite fusarium oxysporum f. Sp. lini

AU - Samsonova, Anastasia

AU - Kanapin, Alexander

AU - Bankin, Michael

AU - Logachev, Anton

AU - Gretsova, Maria

AU - Rozhmina, Tatyana

AU - Samsonova, Maria

N1 - Funding Information: This work was supported by RSF fund, grant 19-16-00030 to A.K., T.R., M.B. and M.S. (data processing, genome annotation, comparative proteomics, plant inoculation, plant and fungi handling, RNA extraction). A.S. and M.G. (functional genomics, genome integrity analyses) were supported by RSF fund, grant 20-14-00072. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/3/6

Y1 - 2021/3/6

N2 - Fusarium wilt of flax is an aggressive disease caused by the soil-borne fungal pathogen Fusarium oxysporum f. sp. lini. It is a challenging pathogen presenting a constant threat to flax production industry worldwide. Previously, we reported chromosome-level assemblies of 5 highly pathogenic F. oxysporum f. sp. lini strains. We sought to characterize the genomic architecture of the fungus and outline evolutionary mechanisms shaping the pathogen genome. Here, we reveal the complex multi-compartmentalized genome organization and uncover its diverse evolutionary dy-namics, which boosts genetic diversity and facilitates host adaptation. In addition, our results suggest that host of functions implicated in the life cycle of mobile genetic elements are main contribu-tors to dissimilarity between proteomes of different Fusaria. Finally, our experiments demonstrate that mobile genetics elements are expressed in planta upon infection, alluding to their role in path-ogenicity. On the whole, these results pave the way for further in-depth studies of evolutionary forces shaping the host–pathogen interaction.

AB - Fusarium wilt of flax is an aggressive disease caused by the soil-borne fungal pathogen Fusarium oxysporum f. sp. lini. It is a challenging pathogen presenting a constant threat to flax production industry worldwide. Previously, we reported chromosome-level assemblies of 5 highly pathogenic F. oxysporum f. sp. lini strains. We sought to characterize the genomic architecture of the fungus and outline evolutionary mechanisms shaping the pathogen genome. Here, we reveal the complex multi-compartmentalized genome organization and uncover its diverse evolutionary dy-namics, which boosts genetic diversity and facilitates host adaptation. In addition, our results suggest that host of functions implicated in the life cycle of mobile genetic elements are main contribu-tors to dissimilarity between proteomes of different Fusaria. Finally, our experiments demonstrate that mobile genetics elements are expressed in planta upon infection, alluding to their role in path-ogenicity. On the whole, these results pave the way for further in-depth studies of evolutionary forces shaping the host–pathogen interaction.

KW - Comparative genomics

KW - Flax

KW - Fusarium oxysporum f. sp. lini

KW - Genome architecture

KW - Genome evolution

KW - Transposable elements

KW - genome evolution

KW - lini

KW - genome architecture

KW - comparative genomics

KW - Fusarium oxysporum f

KW - flax

KW - transposable elements

KW - sp

UR - http://www.scopus.com/inward/record.url?scp=85102005776&partnerID=8YFLogxK

U2 - 10.3390/ijms22052665

DO - 10.3390/ijms22052665

M3 - Article

AN - SCOPUS:85102005776

VL - 22

SP - 1

EP - 16

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 5

M1 - 2665

ER -