TY - JOUR

T1 - A comprehensive study of biocompatibility of detonation nanodiamonds

AU - Berdichevskiy, Gregory M.

AU - Vasina, Lubov V.

AU - Ageev, Sergei V.

AU - Meshcheriakov, Anatolii A.

AU - Galkin, Mikhail A.

AU - Ishmukhametov, Robert R.

AU - Nashchekin, Alexei V.

AU - Kirilenko, Demid A.

AU - Petrov, Andrey V.

AU - Martynova, Sofia D.

AU - Semenov, Konstantin N.

AU - Sharoyko, Vladimir V.

N1 - Publisher Copyright: © 2021 Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/6/15

Y1 - 2021/6/15

N2 - The article describes a complex study of detonation nanodiamonds (DND) aqueous dispersions. In this research, DND sample was characterised by means of IR, NMR spectroscopy, TEM, thermogravimetric analysis, size distribution, and ζ-potentials. It was shown that DND sample includes several surface groups, mainly hydroxylic, carboxylic, and carbonyl ones. Dynamic light scattering results revealed that in the concentration range C = 0.002–0.3 wt%, DND nanoparticles size is equal to 55 ± 5 nm. It was demonstrated that DND possessed weak antiradical activity, had an inhibitory effect on F1F0-ATPase activity, almost did not affect platelet aggregation, formed a stronger complex with human serum albumin (HSA) in subdomain IB (digitoxin, Kb = 20.0 ± 2.4 l·g−1) and a less strong complex in subdomain IIA (warfarin, Kb = 3.7 ± 0.1 l·g−1), inhibited the esterase activity of HSA, DND dispersions (C = 0.0012–0.15 wt%) revealed genotoxic effect towards PBMCs, did not affect cellular proliferation in the experiment with HEK293 cell line, did not reveal cytotoxic effect up to 0.01 wt%. Using DFT and MD approaches allowed us to perform a simulation of interaction between DND nanoparticle and water molecules.

AB - The article describes a complex study of detonation nanodiamonds (DND) aqueous dispersions. In this research, DND sample was characterised by means of IR, NMR spectroscopy, TEM, thermogravimetric analysis, size distribution, and ζ-potentials. It was shown that DND sample includes several surface groups, mainly hydroxylic, carboxylic, and carbonyl ones. Dynamic light scattering results revealed that in the concentration range C = 0.002–0.3 wt%, DND nanoparticles size is equal to 55 ± 5 nm. It was demonstrated that DND possessed weak antiradical activity, had an inhibitory effect on F1F0-ATPase activity, almost did not affect platelet aggregation, formed a stronger complex with human serum albumin (HSA) in subdomain IB (digitoxin, Kb = 20.0 ± 2.4 l·g−1) and a less strong complex in subdomain IIA (warfarin, Kb = 3.7 ± 0.1 l·g−1), inhibited the esterase activity of HSA, DND dispersions (C = 0.0012–0.15 wt%) revealed genotoxic effect towards PBMCs, did not affect cellular proliferation in the experiment with HEK293 cell line, did not reveal cytotoxic effect up to 0.01 wt%. Using DFT and MD approaches allowed us to perform a simulation of interaction between DND nanoparticle and water molecules.

KW - Detonation nanodiamonds

KW - DPPH

KW - Genotoxicity

KW - HSA

KW - Molecular dynamics

KW - Platelets

KW - NUCLEAR-MAGNETIC-RESONANCE

KW - FLUORESCENT NANODIAMONDS

KW - DIAMOND-LIKE CARBON

KW - NANOPARTICLES

KW - POWDERS

KW - SPECTROSCOPY

KW - FTIR

KW - SURFACE

KW - CHEMISTRY

KW - COATINGS

UR - http://www.scopus.com/inward/record.url?scp=85102266989&partnerID=8YFLogxK

U2 - 10.1016/j.molliq.2021.115763

DO - 10.1016/j.molliq.2021.115763

M3 - Article

AN - SCOPUS:85102266989

VL - 332

JO - Journal of Molecular Liquids

JF - Journal of Molecular Liquids

SN - 0167-7322

M1 - 115763

ER -