TY - JOUR

T1 - A chemiluminescence method for screening of fluoroquinolones in milk samples based on a multi-pumping flow system

AU - Vakh, Christina

AU - Pochivalov, Aleksei

AU - Koronkiewicz, Stanislawa

AU - Postnov, Viktor

AU - Bulatov, Andrey

PY - 2019/1/1

Y1 - 2019/1/1

N2 - An automated and miniaturized chemiluminescence method for screening of fluoroquinolones in milk samples was proposed. The method was based on magnetic dispersive micro-solid phase extraction of analytes followed by the chemiluminescence determination of the total fluoroquinolones content using a multi-pumping flow system. In the developed method, Zr-Fe-C magnetic nanoparticles were used as an efficient sorbent for separation of fluoroquinolones from sample matrix. The chemiluminescence intensity obtained for Ce 4+-SO 3 2−-Tb 3+ chemiluminescence system was greatly increased in the presence of the analytes. Under the optimal conditions, the detector response for fluoroquinolones was linear in the concentration ranges from 5·10 −9 to 1·10 −6 mol L −1 with respect to fleroxacin. The limit of detection, calculated from a blank test based on 3σ was 2·10 −9 mol L −1 with respect to fleroxacin. The presented method demonstrated to be a good tool for available and cost-effective point-of-need screening fluoroquinolones in milk samples.

AB - An automated and miniaturized chemiluminescence method for screening of fluoroquinolones in milk samples was proposed. The method was based on magnetic dispersive micro-solid phase extraction of analytes followed by the chemiluminescence determination of the total fluoroquinolones content using a multi-pumping flow system. In the developed method, Zr-Fe-C magnetic nanoparticles were used as an efficient sorbent for separation of fluoroquinolones from sample matrix. The chemiluminescence intensity obtained for Ce 4+-SO 3 2−-Tb 3+ chemiluminescence system was greatly increased in the presence of the analytes. Under the optimal conditions, the detector response for fluoroquinolones was linear in the concentration ranges from 5·10 −9 to 1·10 −6 mol L −1 with respect to fleroxacin. The limit of detection, calculated from a blank test based on 3σ was 2·10 −9 mol L −1 with respect to fleroxacin. The presented method demonstrated to be a good tool for available and cost-effective point-of-need screening fluoroquinolones in milk samples.

KW - Ammonium cerium (IV) nitrate (PubChem CID: 9869224)

KW - Ammonium hydroxide (PubChem CID: 14923)

KW - Chemiluminescence

KW - Direct-injection detector

KW - Fleroxacin (PubChem CID: 3357)

KW - Fluoroquinolones

KW - Lomefloxacin (PubChem CID: 3948)

KW - Magnetic dispersive micro-solid phase extraction

KW - Methanol (PubChem CID: 887)

KW - Milk

KW - Multi-pumping flow system

KW - Norfloxacin (PubChem CID: 4539)

KW - Ofloxacin (PubChem CID: 4583)

KW - Screening

KW - Sodium hydroxide (PubChem CID: 14798)

KW - Sodium sulfite (PubChem CID: 24437)

KW - Terbium chloride hexahydrate (PubChem CID: 203091)

KW - HPLC-FLD DETERMINATION

KW - SULFONAMIDE

KW - OFLOXACIN

KW - SENSITIZED CHEMILUMINESCENCE

KW - SOLID-PHASE EXTRACTION

KW - ASSAY

KW - MEAT

KW - LIQUID-CHROMATOGRAPHY

KW - RESIDUES

UR - http://www.scopus.com/inward/record.url?scp=85049787641&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2018.07.073

DO - 10.1016/j.foodchem.2018.07.073

M3 - Article

VL - 270

SP - 10

EP - 16

JO - Food Chemistry

JF - Food Chemistry

SN - 0308-8146

ER -