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Создание векторов для редактирования генома дрожжей-сахаромицетов на основе системы CRISPR-Cas9. / Матвеенко, А. Г.; Михайличенко, А. С.; Журавлева, Г. А.

в: МИКРОБИОЛОГИЯ, Том 93, № 2, 28.08.2024, стр. 139-144.

Результаты исследований: Научные публикации в периодических изданияхписьмо/краткое сообщениеРецензирование

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@article{c118ccb8b0894302b2905dfbfeea7780,
title = "Создание векторов для редактирования генома дрожжей-сахаромицетов на основе системы CRISPR-Cas9",
abstract = "New vectors for the yeast genome editing using CRISPR/Cas9 were constructed. A system for cloning of new targets using the standard methods (PCR‒restriction‒ligation) was developed and successfully applied. The constructed vectors allowed us to obtain the sup35-25 mutants, deletion of the PSH1 gene and disruption of the NAM7 (UPF1). A convenient method for identifying plasmids with a new target was tested. A detailed description of the cloning technique used and selection of plasmids with the new targets is provided.",
author = "Матвеенко, {А. Г.} and Михайличенко, {А. С.} and Журавлева, {Г. А.}",
year = "2024",
month = aug,
day = "28",
doi = "10.31857/s0026365624020073",
language = "русский",
volume = "93",
pages = "139--144",
journal = "МИКРОБИОЛОГИЯ",
issn = "0026-3656",
publisher = "Издательство {"}Наука{"}",
number = "2",

}

RIS

TY - JOUR

T1 - Создание векторов для редактирования генома дрожжей-сахаромицетов на основе системы CRISPR-Cas9

AU - Матвеенко, А. Г.

AU - Михайличенко, А. С.

AU - Журавлева, Г. А.

PY - 2024/8/28

Y1 - 2024/8/28

N2 - New vectors for the yeast genome editing using CRISPR/Cas9 were constructed. A system for cloning of new targets using the standard methods (PCR‒restriction‒ligation) was developed and successfully applied. The constructed vectors allowed us to obtain the sup35-25 mutants, deletion of the PSH1 gene and disruption of the NAM7 (UPF1). A convenient method for identifying plasmids with a new target was tested. A detailed description of the cloning technique used and selection of plasmids with the new targets is provided.

AB - New vectors for the yeast genome editing using CRISPR/Cas9 were constructed. A system for cloning of new targets using the standard methods (PCR‒restriction‒ligation) was developed and successfully applied. The constructed vectors allowed us to obtain the sup35-25 mutants, deletion of the PSH1 gene and disruption of the NAM7 (UPF1). A convenient method for identifying plasmids with a new target was tested. A detailed description of the cloning technique used and selection of plasmids with the new targets is provided.

UR - https://www.mendeley.com/catalogue/8915cfb8-2731-3270-9b73-6fa48d304e2a/

U2 - 10.31857/s0026365624020073

DO - 10.31857/s0026365624020073

M3 - письмо/краткое сообщение

VL - 93

SP - 139

EP - 144

JO - МИКРОБИОЛОГИЯ

JF - МИКРОБИОЛОГИЯ

SN - 0026-3656

IS - 2

ER -

ID: 127487942