Результаты исследований: Научные публикации в периодических изданиях › письмо/краткое сообщение › Рецензирование
Создание векторов для редактирования генома дрожжей-сахаромицетов на основе системы CRISPR-Cas9. / Матвеенко, А. Г.; Михайличенко, А. С.; Журавлева, Г. А.
в: МИКРОБИОЛОГИЯ, Том 93, № 2, 28.08.2024, стр. 139-144.Результаты исследований: Научные публикации в периодических изданиях › письмо/краткое сообщение › Рецензирование
}
TY - JOUR
T1 - Создание векторов для редактирования генома дрожжей-сахаромицетов на основе системы CRISPR-Cas9
AU - Матвеенко, А. Г.
AU - Михайличенко, А. С.
AU - Журавлева, Г. А.
PY - 2024/8/28
Y1 - 2024/8/28
N2 - New vectors for the yeast genome editing using CRISPR/Cas9 were constructed. A system for cloning of new targets using the standard methods (PCR‒restriction‒ligation) was developed and successfully applied. The constructed vectors allowed us to obtain the sup35-25 mutants, deletion of the PSH1 gene and disruption of the NAM7 (UPF1). A convenient method for identifying plasmids with a new target was tested. A detailed description of the cloning technique used and selection of plasmids with the new targets is provided.
AB - New vectors for the yeast genome editing using CRISPR/Cas9 were constructed. A system for cloning of new targets using the standard methods (PCR‒restriction‒ligation) was developed and successfully applied. The constructed vectors allowed us to obtain the sup35-25 mutants, deletion of the PSH1 gene and disruption of the NAM7 (UPF1). A convenient method for identifying plasmids with a new target was tested. A detailed description of the cloning technique used and selection of plasmids with the new targets is provided.
UR - https://www.mendeley.com/catalogue/8915cfb8-2731-3270-9b73-6fa48d304e2a/
U2 - 10.31857/s0026365624020073
DO - 10.31857/s0026365624020073
M3 - письмо/краткое сообщение
VL - 93
SP - 139
EP - 144
JO - МИКРОБИОЛОГИЯ
JF - МИКРОБИОЛОГИЯ
SN - 0026-3656
IS - 2
ER -
ID: 127487942