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DOI

New vectors for the yeast genome editing using CRISPR/Cas9 were constructed. A system for cloning of new targets using the standard methods (PCR‒restriction‒ligation) was developed and successfully applied. The constructed vectors allowed us to obtain the sup35-25 mutants, deletion of the PSH1 gene and disruption of the NAM7 (UPF1). A convenient method for identifying plasmids with a new target was tested. A detailed description of the cloning technique used and selection of plasmids with the new targets is provided.
Переведенное названиеConstruction of Vectors for the Genome Editing of Saccharomyces Yeast Using CRISPR-Cas9 System
Язык оригиналарусский
Страницы (с-по)139-144
Число страниц6
ЖурналМИКРОБИОЛОГИЯ
Том93
Номер выпуска2
DOI
СостояниеОпубликовано - 28 авг 2024

ID: 127487942