TY - JOUR

T1 - Некоторые особенности определения индексов удерживания в обращенно-фазовой ВЭЖХ

AU - Зенкевич, Игорь Георгиевич

AU - Деруиш, Абденнур

AU - Никитина, Дарья Александровна

N1 - Funding Information: Experimental data of current work with methanol as component of an eluent were performed at the Center for Chemical and Material Researches of Research Park of St. Petersburg State University. The authors are grateful to the staff of this Center for assistance. Publisher Copyright: © 2022 Ural Federal University. All rights reserved.

PY - 2022/3

Y1 - 2022/3

N2 - The features of the retention indices determination in reversed phase high performance liquid chromatography (RP HPLC) are discussed, namely the different possibilities of the application of the recurrent relations. At first, not the experimental, but the precalculated values are recommended as the retention parameters of non-sorbable compound (dead time, t 0) in RP HPLC. It is because just the precalculated values provide the linearity of relationships lg (t R – t 0) = an C + b, where n C is the number of carbon atoms in molecules of homologs selected as reference compounds (n-alkyl phenyl ketones). Besides the known Peterson and Hirsch equation for the theoretical evaluation of t 0 using the retention times of three consecutive homologs, the following recurrent relations are also recommended: t R(n С – 1) = at R(n C) + b, from where it follows that t 0 = lim (t R)| (n C → 0) = b / (1 – a) under the condition that 0 < a < 1. Another area of the effective application of the recurrent relations is caused by the following fact: the ranges of the concentrations of the eluent’s organic constituents during the separation of hydrophilic analytes and hydrophobic reference n-alkyl phenyl ketones may not overlap, or overlap only partially. In such cases, expanding the data arrays seems to be reasonable using the extrapolated values both for the target analytes, and (preferably) for the reference compounds. For these purposes, another kind of recurrent relations can be recommended, namely t R(C ± ΔC) = at R(C) + b, ΔC = const. All recommendations on the application of recurrent relations in RP HPLC are illustrated with examples.

AB - The features of the retention indices determination in reversed phase high performance liquid chromatography (RP HPLC) are discussed, namely the different possibilities of the application of the recurrent relations. At first, not the experimental, but the precalculated values are recommended as the retention parameters of non-sorbable compound (dead time, t 0) in RP HPLC. It is because just the precalculated values provide the linearity of relationships lg (t R – t 0) = an C + b, where n C is the number of carbon atoms in molecules of homologs selected as reference compounds (n-alkyl phenyl ketones). Besides the known Peterson and Hirsch equation for the theoretical evaluation of t 0 using the retention times of three consecutive homologs, the following recurrent relations are also recommended: t R(n С – 1) = at R(n C) + b, from where it follows that t 0 = lim (t R)| (n C → 0) = b / (1 – a) under the condition that 0 < a < 1. Another area of the effective application of the recurrent relations is caused by the following fact: the ranges of the concentrations of the eluent’s organic constituents during the separation of hydrophilic analytes and hydrophobic reference n-alkyl phenyl ketones may not overlap, or overlap only partially. In such cases, expanding the data arrays seems to be reasonable using the extrapolated values both for the target analytes, and (preferably) for the reference compounds. For these purposes, another kind of recurrent relations can be recommended, namely t R(C ± ΔC) = at R(C) + b, ΔC = const. All recommendations on the application of recurrent relations in RP HPLC are illustrated with examples.

KW - В тексте статьи

KW - Reversed-phase high performance liquid chromatography

KW - Retention indices

KW - Reference n-alkyl phenyl ketones

KW - Important features of determination and calculation

UR - http://www.scopus.com/inward/record.url?scp=85129732636&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/3ea81817-5288-34d3-a2ef-63215e4ee6e5/

U2 - 10.15826/analitika.2022.26.1.007

DO - 10.15826/analitika.2022.26.1.007

M3 - статья

VL - 26

SP - 57

EP - 63

JO - АНАЛИТИКА И КОНТРОЛЬ

JF - АНАЛИТИКА И КОНТРОЛЬ

SN - 2073-1442

IS - 1

ER -