Transcriptomic analysis of radish (Raphanus sativus l.) spontaneous tumor

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Transcriptomic analysis of radish (Raphanus sativus l.) spontaneous tumor. / Tkachenko, Alexander; Dodueva, Irina ; Tvorogova, Varvara; Predeus, Alexander; Pravdina, Olga ; Kuznetsova, Ksenia ; Lutova, Ludmila.

In: Plants, Vol. 10, No. 5, 919, 05.2021.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{257e017cd5234d30bde8fef384ee183a,

title = "Transcriptomic analysis of radish (Raphanus sativus l.) spontaneous tumor",

abstract = "Spontaneous tumors can develop in different organs of various plant species without any pathogen infection and, as a rule, appear in plants with a certain genotype: Mutants, interspecific hybrids, etc. In particular, among the inbred lines of radish (Raphanus sativus L.), lines that form spontaneous tumors on the taproot during the flowering period were obtained many years ago. In this work, we analyzed the differential gene expression in the spontaneous tumors of radish versus the lateral roots using the RNA-seq method. Data were obtained indicating the increased expression of genes associated with cell division and growth (especially genes that regulate G2-M transition and cytokinesis) in the spontaneous tumor. Among genes downregulated in the tumor tissue, genes participating in the response to stress and wounding, mainly involved in the biosynthesis of jasmonic acid and glucosinolates, were enriched. Our data will help elucidate the mechanisms of spontaneous tumor development in higher plants.",

keywords = "Cell division, Differential gene expression, Raphanus sativus, RNA-seq, Spontaneous tumor, Stress response, ANALYSIS REVEALS, RESPONSE REGULATORS, INBRED LINES, cell division, spontaneous tumor, CIRCADIAN CLOCK, differential gene expression, IN-VITRO, stress response, CELL PLATE FORMATION, GLUCOSINOLATE BIOSYNTHESIS, LATERAL ROOT-FORMATION, CYCLIN-DEPENDENT-KINASES, JASMONIC ACID",

author = "Alexander Tkachenko and Irina Dodueva and Varvara Tvorogova and Alexander Predeus and Olga Pravdina and Ksenia Kuznetsova and Ludmila Lutova",

year = "2021",

month = may,

doi = "10.3390/plants10050919",

language = "English",

volume = "10",

journal = "Plants",

issn = "2223-7747",

publisher = "MDPI AG",

number = "5",

}

RIS

TY - JOUR

T1 - Transcriptomic analysis of radish (Raphanus sativus l.) spontaneous tumor

AU - Tkachenko, Alexander

AU - Dodueva, Irina

AU - Tvorogova, Varvara

AU - Predeus, Alexander

AU - Pravdina, Olga

AU - Kuznetsova, Ksenia

AU - Lutova, Ludmila

PY - 2021/5

Y1 - 2021/5

N2 - Spontaneous tumors can develop in different organs of various plant species without any pathogen infection and, as a rule, appear in plants with a certain genotype: Mutants, interspecific hybrids, etc. In particular, among the inbred lines of radish (Raphanus sativus L.), lines that form spontaneous tumors on the taproot during the flowering period were obtained many years ago. In this work, we analyzed the differential gene expression in the spontaneous tumors of radish versus the lateral roots using the RNA-seq method. Data were obtained indicating the increased expression of genes associated with cell division and growth (especially genes that regulate G2-M transition and cytokinesis) in the spontaneous tumor. Among genes downregulated in the tumor tissue, genes participating in the response to stress and wounding, mainly involved in the biosynthesis of jasmonic acid and glucosinolates, were enriched. Our data will help elucidate the mechanisms of spontaneous tumor development in higher plants.

AB - Spontaneous tumors can develop in different organs of various plant species without any pathogen infection and, as a rule, appear in plants with a certain genotype: Mutants, interspecific hybrids, etc. In particular, among the inbred lines of radish (Raphanus sativus L.), lines that form spontaneous tumors on the taproot during the flowering period were obtained many years ago. In this work, we analyzed the differential gene expression in the spontaneous tumors of radish versus the lateral roots using the RNA-seq method. Data were obtained indicating the increased expression of genes associated with cell division and growth (especially genes that regulate G2-M transition and cytokinesis) in the spontaneous tumor. Among genes downregulated in the tumor tissue, genes participating in the response to stress and wounding, mainly involved in the biosynthesis of jasmonic acid and glucosinolates, were enriched. Our data will help elucidate the mechanisms of spontaneous tumor development in higher plants.

KW - Cell division

KW - Differential gene expression

KW - Raphanus sativus

KW - RNA-seq

KW - Spontaneous tumor

KW - Stress response

KW - ANALYSIS REVEALS

KW - RESPONSE REGULATORS

KW - INBRED LINES

KW - cell division

KW - spontaneous tumor

KW - CIRCADIAN CLOCK

KW - differential gene expression

KW - IN-VITRO

KW - stress response

KW - CELL PLATE FORMATION

KW - GLUCOSINOLATE BIOSYNTHESIS

KW - LATERAL ROOT-FORMATION

KW - CYCLIN-DEPENDENT-KINASES

KW - JASMONIC ACID

UR - http://www.scopus.com/inward/record.url?scp=85105015927&partnerID=8YFLogxK

U2 - 10.3390/plants10050919

DO - 10.3390/plants10050919

M3 - Article

AN - SCOPUS:85105015927

VL - 10

JO - Plants

JF - Plants

SN - 2223-7747

IS - 5

M1 - 919

ER -

ID: 76837844