Stir flat sheet membrane liquid phase microextraction for the selective chemiluminescence determination of ofloxacin and fleroxacin in human urine

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Stir flat sheet membrane liquid phase microextraction for the selective chemiluminescence determination of ofloxacin and fleroxacin in human urine. / Vakh, Christina ; Likanov, Gleb ; Bulatov, Andrey.

In: Microchemical Journal, Vol. 163, 105913, 04.2021.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{19b2df2386a949aa9979b2aef4eb4bab,

title = "Stir flat sheet membrane liquid phase microextraction for the selective chemiluminescence determination of ofloxacin and fleroxacin in human urine",

abstract = "Stir flat sheet membrane liquid phase microextraction procedure for the chemiluminescence determination of polar antibiotics (ofloxacin and fleroxacin) in human urine samples was developed. The microextraction procedure assumed simultaneous ion-pair complex formation between antibiotic and anionic dye in acidic medium, dispersion of extraction solvent by rotation of a stir flat sheet microporous membrane unit, mass-transfer of complexes from sample into microdroplets of extraction solvent followed by extract collection into the unit. After preconcentration the unit was immersed in alkaline aqueous solution for analytes re-extraction based on ion-pair complex decomposition followed by the chemiluminescence analysis of obtained aqueous phase. The developed microextraction procedure allowed to exclude matrix effects for the selective, simple, and rapid chemiluminescence determination of ofloxacin and fleroxacin in urine samples. The calibration graphs were linear over the concentration ranges of 0.1–25 mg L−1 for ofloxacin and fleroxacin. The LOD values, calculated from the blank tests based on 3σ, were 30 µg L−1 for ofloxacin and fleroxacin. The procedure was conveniently applied to the determination of fluoroquinolones in urine samples.",

keywords = "Chemiluminescence, Fluoroquinolones, Ion-pair complex, Membrane-based liquid phase microextraction, Stir flat sheet membrane unit, Urine, PHARMACEUTICALS, LOMEFLOXACIN",

author = "Christina Vakh and Gleb Likanov and Andrey Bulatov",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

year = "2021",

month = apr,

doi = "10.1016/j.microc.2020.105913",

language = "English",

volume = "163",

journal = "Microchemical Journal",

issn = "0026-265X",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Stir flat sheet membrane liquid phase microextraction for the selective chemiluminescence determination of ofloxacin and fleroxacin in human urine

AU - Vakh, Christina

AU - Likanov, Gleb

AU - Bulatov, Andrey

PY - 2021/4

Y1 - 2021/4

N2 - Stir flat sheet membrane liquid phase microextraction procedure for the chemiluminescence determination of polar antibiotics (ofloxacin and fleroxacin) in human urine samples was developed. The microextraction procedure assumed simultaneous ion-pair complex formation between antibiotic and anionic dye in acidic medium, dispersion of extraction solvent by rotation of a stir flat sheet microporous membrane unit, mass-transfer of complexes from sample into microdroplets of extraction solvent followed by extract collection into the unit. After preconcentration the unit was immersed in alkaline aqueous solution for analytes re-extraction based on ion-pair complex decomposition followed by the chemiluminescence analysis of obtained aqueous phase. The developed microextraction procedure allowed to exclude matrix effects for the selective, simple, and rapid chemiluminescence determination of ofloxacin and fleroxacin in urine samples. The calibration graphs were linear over the concentration ranges of 0.1–25 mg L−1 for ofloxacin and fleroxacin. The LOD values, calculated from the blank tests based on 3σ, were 30 µg L−1 for ofloxacin and fleroxacin. The procedure was conveniently applied to the determination of fluoroquinolones in urine samples.

AB - Stir flat sheet membrane liquid phase microextraction procedure for the chemiluminescence determination of polar antibiotics (ofloxacin and fleroxacin) in human urine samples was developed. The microextraction procedure assumed simultaneous ion-pair complex formation between antibiotic and anionic dye in acidic medium, dispersion of extraction solvent by rotation of a stir flat sheet microporous membrane unit, mass-transfer of complexes from sample into microdroplets of extraction solvent followed by extract collection into the unit. After preconcentration the unit was immersed in alkaline aqueous solution for analytes re-extraction based on ion-pair complex decomposition followed by the chemiluminescence analysis of obtained aqueous phase. The developed microextraction procedure allowed to exclude matrix effects for the selective, simple, and rapid chemiluminescence determination of ofloxacin and fleroxacin in urine samples. The calibration graphs were linear over the concentration ranges of 0.1–25 mg L−1 for ofloxacin and fleroxacin. The LOD values, calculated from the blank tests based on 3σ, were 30 µg L−1 for ofloxacin and fleroxacin. The procedure was conveniently applied to the determination of fluoroquinolones in urine samples.

KW - Chemiluminescence

KW - Fluoroquinolones

KW - Ion-pair complex

KW - Membrane-based liquid phase microextraction

KW - Stir flat sheet membrane unit

KW - Urine

KW - PHARMACEUTICALS

KW - LOMEFLOXACIN

UR - http://www.scopus.com/inward/record.url?scp=85100206931&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/d1964eac-a8b9-3578-8dd9-f526b07d2c1e/

U2 - 10.1016/j.microc.2020.105913

DO - 10.1016/j.microc.2020.105913

M3 - Article

AN - SCOPUS:85100206931

VL - 163

JO - Microchemical Journal

JF - Microchemical Journal

SN - 0026-265X

M1 - 105913

ER -

ID: 87512342