TY - JOUR

T1 - Silk Fibroin Self-Assembly at the Air–Water Interface

AU - Акентьев, Александр Владимирович

AU - Быков, Алексей Геннадьевич

AU - Миллер, Райнхард

AU - Рафикова, Анастасия Рамильевна

AU - Ротанова, Ксения Юрьевна

AU - Носков, Борис Анатольевич

A2 - Миляева, Ольга Юрьевна

PY - 2024/6/2

Y1 - 2024/6/2

N2 - Amphiphilic silk fibroin (SF) forms stable adsorption layers at the air–water interface. The range of the investigated protein concentrations can be divided into two parts according to the peculiarities of the surface layer properties. At protein concentrations from 0.0005 to 0.01 mg/mL, the dynamic surface elasticity monotonically increases with the concentration and surface age and reaches values of up to 220 mN/m. In this range, the adsorption layer compression leads to a fast increase of the surface pressure. In the second part (>0.01 mg/mL), the surface elasticity decreases again and the kinetic dependences of the film thickness and adsorbed amount change only a little. In this case, the layer compression leads only to a slight increase of the surface pressure. These two types of behavior can be attributed to the distinctions in the protein aggregation in the surface layer. Atomic force microscopy (AFM) investigations of the layers transferred from the liquid surface onto a mica surface by the Langmuir–Schaefer method show some peculiarities of the layer morphology in the intermediate concentration range (~0.02 mg/mL).

AB - Amphiphilic silk fibroin (SF) forms stable adsorption layers at the air–water interface. The range of the investigated protein concentrations can be divided into two parts according to the peculiarities of the surface layer properties. At protein concentrations from 0.0005 to 0.01 mg/mL, the dynamic surface elasticity monotonically increases with the concentration and surface age and reaches values of up to 220 mN/m. In this range, the adsorption layer compression leads to a fast increase of the surface pressure. In the second part (>0.01 mg/mL), the surface elasticity decreases again and the kinetic dependences of the film thickness and adsorbed amount change only a little. In this case, the layer compression leads only to a slight increase of the surface pressure. These two types of behavior can be attributed to the distinctions in the protein aggregation in the surface layer. Atomic force microscopy (AFM) investigations of the layers transferred from the liquid surface onto a mica surface by the Langmuir–Schaefer method show some peculiarities of the layer morphology in the intermediate concentration range (~0.02 mg/mL).

KW - фиброин шелка

KW - граница раздела воздух–вода

KW - фибриллы

KW - адсорбционные слои

KW - дилатационная поверхностная вязкоупругость

KW - эллипсометрия

KW - толщина слоя

UR - https://www.mendeley.com/catalogue/adfff6bd-0d93-36a4-8ad3-c53b7ba482c5/

U2 - 10.3390/colloids8030035

DO - 10.3390/colloids8030035

M3 - Article

VL - 8

SP - 35

JO - Colloids and Interfaces

JF - Colloids and Interfaces

SN - 2504-5377

IS - 3

M1 - 35

ER -