A simple and efficient technique for voltammetric enzymeless detection of epinephrine (EP) was proposed. In this technique, we applied hierarchical Ru-based electrode modified by Au nano- to micro-sized particles that was fabricated using laser-assisted synthesis. The cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed to characterize electrochemical properties of the fabricated electrode material. For EP detection, we obtained two DPV calibration curves that were linear in the range of 0.05–5 μM and 5–500 μM. The highest sensitivity (45.0 μA μM−1 cm−2) and the lowest detection limit (6.4 nM) were observed for the first linear range, whereas the estimated sensitivity and limit of detection for the second linear range were 2.0 μA μM−1 cm−2 and 18.7 nM, respectively. We also demonstrated that the proposed technique can be used for selective EP determination in the presence of such common interfering analytes as ascorbic acid and dopamine. The results of this study can be potentially used for development of low-cost voltammetric sensor platforms for non-enzymatic epinephrine detection.