Raman spectroscopy for glutathione measurements in Mycobacterium tuberculosis strains with different antibiotic resistance

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Raman spectroscopy for glutathione measurements in Mycobacterium tuberculosis strains with different antibiotic resistance. / Zyubin, Andrey; Lavrova, Anastasia; Manicheva, Olga; Dogonadze, Marine; Belik, Vitaly; Samusev, Ilya.

In: Journal of Raman Spectroscopy, Vol. 52, No. 9, 09.2021, p. 1661-1666.

Research output: Contribution to journal › Article › peer-review

Author

Zyubin, Andrey ; Lavrova, Anastasia ; Manicheva, Olga ; Dogonadze, Marine ; Belik, Vitaly ; Samusev, Ilya. / Raman spectroscopy for glutathione measurements in Mycobacterium tuberculosis strains with different antibiotic resistance. In: Journal of Raman Spectroscopy. 2021 ; Vol. 52, No. 9. pp. 1661-1666.

BibTeX

@article{39541f928eea4ae5a7ac3a16f01d2ede,

title = "Raman spectroscopy for glutathione measurements in Mycobacterium tuberculosis strains with different antibiotic resistance",

abstract = "The Mycobacterium tuberculosis (MbT) drug resistance remains a serious problem to global public health and usually leads to a lethal outcome in patients with such strain MbT. Precise and rapid identification of a specific MbT strain is vital for the appointment of anti-tuberculosis therapy. We continue investigation of deactivated MbT strains of the Beijing family by Raman spectroscopy method and focus on glutathione (GSH) as a potential biomarker for strains identification. Strains under the study have different drug sensitivity: sensitive, multi, and extensively drug resistant. Samples were investigated by Raman spectrometry with He-Ne (633 nm) laser excitation source in the “fingerprint” region. As a result, GSH vibrational bands for potential strain discrimination were determined. These differences can be described for drug-sensitive and drug-resistant strains taken of pulmonary and extra-pulmonary forms of tuberculosis. We suppose that the obtained results can be useful for new diagnostic tools development for identification of different MbT strains in clinical medicine and research practice.",

keywords = "bacteria, cell-wall, glutathione, mycobacterium tuberculosis, raman spectroscopy",

author = "Andrey Zyubin and Anastasia Lavrova and Olga Manicheva and Marine Dogonadze and Vitaly Belik and Ilya Samusev",

note = "Publisher Copyright: {\textcopyright} 2021 John Wiley & Sons, Ltd.",

year = "2021",

month = sep,

doi = "10.1002/jrs.6088",

language = "English",

volume = "52",

pages = "1661--1666",

journal = "Journal of Raman Spectroscopy",

issn = "0377-0486",

publisher = "Wiley-Blackwell",

number = "9",

}

RIS

TY - JOUR

T1 - Raman spectroscopy for glutathione measurements in Mycobacterium tuberculosis strains with different antibiotic resistance

AU - Zyubin, Andrey

AU - Lavrova, Anastasia

AU - Manicheva, Olga

AU - Dogonadze, Marine

AU - Belik, Vitaly

AU - Samusev, Ilya

PY - 2021/9

Y1 - 2021/9

N2 - The Mycobacterium tuberculosis (MbT) drug resistance remains a serious problem to global public health and usually leads to a lethal outcome in patients with such strain MbT. Precise and rapid identification of a specific MbT strain is vital for the appointment of anti-tuberculosis therapy. We continue investigation of deactivated MbT strains of the Beijing family by Raman spectroscopy method and focus on glutathione (GSH) as a potential biomarker for strains identification. Strains under the study have different drug sensitivity: sensitive, multi, and extensively drug resistant. Samples were investigated by Raman spectrometry with He-Ne (633 nm) laser excitation source in the “fingerprint” region. As a result, GSH vibrational bands for potential strain discrimination were determined. These differences can be described for drug-sensitive and drug-resistant strains taken of pulmonary and extra-pulmonary forms of tuberculosis. We suppose that the obtained results can be useful for new diagnostic tools development for identification of different MbT strains in clinical medicine and research practice.

AB - The Mycobacterium tuberculosis (MbT) drug resistance remains a serious problem to global public health and usually leads to a lethal outcome in patients with such strain MbT. Precise and rapid identification of a specific MbT strain is vital for the appointment of anti-tuberculosis therapy. We continue investigation of deactivated MbT strains of the Beijing family by Raman spectroscopy method and focus on glutathione (GSH) as a potential biomarker for strains identification. Strains under the study have different drug sensitivity: sensitive, multi, and extensively drug resistant. Samples were investigated by Raman spectrometry with He-Ne (633 nm) laser excitation source in the “fingerprint” region. As a result, GSH vibrational bands for potential strain discrimination were determined. These differences can be described for drug-sensitive and drug-resistant strains taken of pulmonary and extra-pulmonary forms of tuberculosis. We suppose that the obtained results can be useful for new diagnostic tools development for identification of different MbT strains in clinical medicine and research practice.

KW - bacteria

KW - cell-wall

KW - glutathione

KW - mycobacterium tuberculosis

KW - raman spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=85101923156&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/42012ae1-4b32-34d0-b3c2-b68568d9d615/

U2 - 10.1002/jrs.6088

DO - 10.1002/jrs.6088

M3 - Article

AN - SCOPUS:85101923156

VL - 52

SP - 1661

EP - 1666

JO - Journal of Raman Spectroscopy

JF - Journal of Raman Spectroscopy

SN - 0377-0486

IS - 9

ER -

ID: 88551369