The Mycobacterium tuberculosis (MbT) drug resistance remains a serious problem to global public health and usually leads to a lethal outcome in patients with such strain MbT. Precise and rapid identification of a specific MbT strain is vital for the appointment of anti-tuberculosis therapy. We continue investigation of deactivated MbT strains of the Beijing family by Raman spectroscopy method and focus on glutathione (GSH) as a potential biomarker for strains identification. Strains under the study have different drug sensitivity: sensitive, multi, and extensively drug resistant. Samples were investigated by Raman spectrometry with He-Ne (633 nm) laser excitation source in the “fingerprint” region. As a result, GSH vibrational bands for potential strain discrimination were determined. These differences can be described for drug-sensitive and drug-resistant strains taken of pulmonary and extra-pulmonary forms of tuberculosis. We suppose that the obtained results can be useful for new diagnostic tools development for identification of different MbT strains in clinical medicine and research practice.