Research output: Contribution to journal › Article › peer-review
Pyrazole derivative attenuates store-dependent Ca2+ entry in rat peritoneal macrophages. / Миленина, Лидия Сергеевна; Крутецкая, Зоя Иринарховна; Антонов, Виктор ; Крутецкая, Нина Иринарховна.
In: Cell and Tissue Biology, Vol. 15, No. 3, 05.2021, p. 293-300.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Pyrazole derivative attenuates store-dependent Ca2+ entry in rat peritoneal macrophages
AU - Миленина, Лидия Сергеевна
AU - Крутецкая, Зоя Иринарховна
AU - Антонов, Виктор
AU - Крутецкая, Нина Иринарховна
N1 - Milenina L.S., Krutetskaya Z.I., Antonov V.G., Krutetskaya N.I. Pyrazole derivative attenuates store-dependent Ca2+ entry in rat peritoneal macrophages. Cell and Tissue Biology. 2021 V. 15. N. 3. P. 181-188.
PY - 2021/5
Y1 - 2021/5
N2 - Abstract—: Store-dependent Ca 2+ entry is a ubiquitous mechanism of regulated Ca 2+ entry into eukaryotic cells. It is activated upon depletion of intracellular Ca 2+ stores and is involved in the regulation of a wide range of cellular processes. To elucidate the pharmacological characteristics of store-dependent Ca 2+ entry into cells, we studied the effect of YM-58483, pyrazole derivative immunosuppressant, on the store-dependent Ca 2+ entry in rat peritoneal macrophages induced by the endoplasmic Ca 2+-ATPase inhibitors thapsigargin and cyclopiazonic acid, as well as the disulfide-containing immunomodulators glutoxim and molixan. Using Fura-2AM, fluorescent calcium indicator, it has been shown for the first time that, in rat peritoneal macrophages, as well as in other cell types, pyrazole derivative YM-58483 effectively inhibits store-dependent Ca 2+ entry and is a useful pharmacological tool to study the store-dependent Ca 2+ entry in macrophages. The data obtained additionally confirm that Ca 2+ entry induced by glutoxim or molixan is realized via the store-dependent mechanism.
AB - Abstract—: Store-dependent Ca 2+ entry is a ubiquitous mechanism of regulated Ca 2+ entry into eukaryotic cells. It is activated upon depletion of intracellular Ca 2+ stores and is involved in the regulation of a wide range of cellular processes. To elucidate the pharmacological characteristics of store-dependent Ca 2+ entry into cells, we studied the effect of YM-58483, pyrazole derivative immunosuppressant, on the store-dependent Ca 2+ entry in rat peritoneal macrophages induced by the endoplasmic Ca 2+-ATPase inhibitors thapsigargin and cyclopiazonic acid, as well as the disulfide-containing immunomodulators glutoxim and molixan. Using Fura-2AM, fluorescent calcium indicator, it has been shown for the first time that, in rat peritoneal macrophages, as well as in other cell types, pyrazole derivative YM-58483 effectively inhibits store-dependent Ca 2+ entry and is a useful pharmacological tool to study the store-dependent Ca 2+ entry in macrophages. The data obtained additionally confirm that Ca 2+ entry induced by glutoxim or molixan is realized via the store-dependent mechanism.
KW - YM-58483 pyrazole derivative
KW - peritoneal macrophages
KW - store-dependent Ca entry
KW - store-dependent Ca2+ entry
UR - http://www.scopus.com/inward/record.url?scp=85108063415&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/b6656545-0f3f-3190-8ce6-f29f98db5781/
U2 - 10.1134/s1990519x21030068
DO - 10.1134/s1990519x21030068
M3 - Article
VL - 15
SP - 293
EP - 300
JO - Cell and Tissue Biology
JF - Cell and Tissue Biology
SN - 1990-519X
IS - 3
ER -
ID: 76343468