Abstract—: Store-dependent Ca ²⁺ entry is a ubiquitous mechanism of regulated Ca ²⁺ entry into eukaryotic cells. It is activated upon depletion of intracellular Ca ²⁺ stores and is involved in the regulation of a wide range of cellular processes. To elucidate the pharmacological characteristics of store-dependent Ca ²⁺ entry into cells, we studied the effect of YM-58483, pyrazole derivative immunosuppressant, on the store-dependent Ca ²⁺ entry in rat peritoneal macrophages induced by the endoplasmic Ca ²⁺-ATPase inhibitors thapsigargin and cyclopiazonic acid, as well as the disulfide-containing immunomodulators glutoxim and molixan. Using Fura-2AM, fluorescent calcium indicator, it has been shown for the first time that, in rat peritoneal macrophages, as well as in other cell types, pyrazole derivative YM-58483 effectively inhibits store-dependent Ca ²⁺ entry and is a useful pharmacological tool to study the store-dependent Ca ²⁺ entry in macrophages. The data obtained additionally confirm that Ca ²⁺ entry induced by glutoxim or molixan is realized via the store-dependent mechanism.