The study considers psycho-emotional well-being as an integrative construct, consisting of personal, cognitive-evaluative and emotional components. The aim is to examine the level and structure of psycho-emotional well-being of adolescents taking into consideration their gender. Within the study 190 persons aged 14-17 (73 boys and 117 girls) were screened using a complex variety of methods to evaluate their psycho-emotional well-being from various points of view. With lowered competency indexes and environment control, the study identified the level of personal well-being as average. Girls were more oriented towards positive interpersonal relations, while boys were more interested in self-development. Cognitive-evaluative indexes demonstrate insufficient satisfaction of respondents with romantic relationships. Further differences were revealed in higher satisfaction of male adolescents with recreational activities, and higher satisfaction of female adolescents with social and legal security. Girls also give a higher mark to the level of need satisfaction. The structure of emotional sphere reveals an increase in the asthenic component of anxiety and emotional discomfort, which are signals of fatigue, stress and tension in all respondents, girls demonstrating higher degree of these symptoms. Correlation analysis proved the hypothesis of a high integration among all the indexes of psycho-emotional well-being. Gender differences in the structure result from the fact that male adolescents attach more importance to self-development for their psycho-emotional well-being, while female adolescents value positive relationships, need and life satisfaction.