Research output: Contribution to journal › Article › peer-review
Probing the conjugation of gold NPs using single- stranded DNA comprising two polyadenine adhesive tails. / Соколов, Петр Александрович; Рамазанов, Руслан Рафядинович.
In: Journal of Adhesion, 06.03.2022.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Probing the conjugation of gold NPs using single- stranded DNA comprising two polyadenine adhesive tails
AU - Соколов, Петр Александрович
AU - Рамазанов, Руслан Рафядинович
N1 - Publisher Copyright: © 2022 Taylor & Francis Group, LLC.
PY - 2022/3/6
Y1 - 2022/3/6
N2 - The spatial arrangement of nanoobjects by using DNA is a promising nano-design technology. Polyadenine adhesive tails are increasingly used to anchor functional oligonucleotides on the surface of gold nanoparticles at low pH. Further hybridization of such functional oligonucleotides allows nanoparticles to be organized within the desired design. In this work, we studied the effect of temperature, ionic strength, and strand design on the adsorption rate of polyadenines on the surface of gold nanoparticles at different pH. We found that there are temperature and ionic strength ranges in which the adsorption rate increases and vice versa. There are also significant differences in the effects of monovalent sodium and divalent magnesium ions. We have shown that dual-tail DNA strand design allows for increased surface density, which is significantly higher than that obtained with the standard one-tail strand design.
AB - The spatial arrangement of nanoobjects by using DNA is a promising nano-design technology. Polyadenine adhesive tails are increasingly used to anchor functional oligonucleotides on the surface of gold nanoparticles at low pH. Further hybridization of such functional oligonucleotides allows nanoparticles to be organized within the desired design. In this work, we studied the effect of temperature, ionic strength, and strand design on the adsorption rate of polyadenines on the surface of gold nanoparticles at different pH. We found that there are temperature and ionic strength ranges in which the adsorption rate increases and vice versa. There are also significant differences in the effects of monovalent sodium and divalent magnesium ions. We have shown that dual-tail DNA strand design allows for increased surface density, which is significantly higher than that obtained with the standard one-tail strand design.
KW - ATP aptamer
KW - DNA
KW - biosensor
KW - conjugation
KW - gold nanoparticles
KW - polyadenine
KW - FUNCTIONALIZATION
KW - NANOPARTICLES
KW - NONTHIOLATED DNA
KW - ADSORPTION
UR - http://www.scopus.com/inward/record.url?scp=85126046487&partnerID=8YFLogxK
UR - https://www.tandfonline.com/doi/full/10.1080/00218464.2022.2047942
UR - https://www.mendeley.com/catalogue/0a705c72-fa44-33cf-aaf2-de451371a861/
U2 - 10.1080/00218464.2022.2047942
DO - 10.1080/00218464.2022.2047942
M3 - Article
JO - Journal of Adhesion
JF - Journal of Adhesion
SN - 0021-8464
ER -
ID: 93245780