TY - JOUR

T1 - Model of cooperative interaction of TMPyP4 porphyrine with DNA quadruplex

AU - Kudrev, A. G.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Intrinsic binding constants of the TMPyP4 porphyrin with G-quadruplex of DNA (ckit2) have been calculated; they can be applied as reference for quantitative evaluation of the possibility of such structures participation in biochemical processes. The conclusion about existence of two different sites of the TMPyP4 binding to ckit2 binding made earlier on the basis of the stepwise complex formation model has been revised using the model of mutual influence (cooperativity) of the ligands bound to the oligonucleotide. The model has been verified using principal component analysis and factor analysis methods. The ckit2 biomolecule can be considered as an oligonucleotide with equivalent mutually affecting binding sites.

AB - Intrinsic binding constants of the TMPyP4 porphyrin with G-quadruplex of DNA (ckit2) have been calculated; they can be applied as reference for quantitative evaluation of the possibility of such structures participation in biochemical processes. The conclusion about existence of two different sites of the TMPyP4 binding to ckit2 binding made earlier on the basis of the stepwise complex formation model has been revised using the model of mutual influence (cooperativity) of the ligands bound to the oligonucleotide. The model has been verified using principal component analysis and factor analysis methods. The ckit2 biomolecule can be considered as an oligonucleotide with equivalent mutually affecting binding sites.

KW - cooperativity

KW - DNA

KW - G-quadruplex

KW - TMPyP4

UR - http://www.scopus.com/inward/record.url?scp=84982732861&partnerID=8YFLogxK

U2 - 10.1134/S1070363216060219

DO - 10.1134/S1070363216060219

M3 - Article

AN - SCOPUS:84982732861

VL - 86

SP - 1353

EP - 1363

JO - Russian Journal of General Chemistry

JF - Russian Journal of General Chemistry

SN - 1070-3632

IS - 6

ER -