Method for obtaining root nodules of the Baikal relict legumes in laboratory pot experiments. / Safronova, Vera; Belimov, Andrey; Andronov, Evgeny; Popova, Janna; Tikhomirova, Nina; Orlova, Olga; Verkhozina, Alla; Chimitov, Daba; Tikhonovich, Igor.
In: International Journal of Environmental Studies, Vol. 74, No. 5, 03.09.2017, p. 700-705.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Method for obtaining root nodules of the Baikal relict legumes in laboratory pot experiments
AU - Safronova, Vera
AU - Belimov, Andrey
AU - Andronov, Evgeny
AU - Popova, Janna
AU - Tikhomirova, Nina
AU - Orlova, Olga
AU - Verkhozina, Alla
AU - Chimitov, Daba
AU - Tikhonovich, Igor
PY - 2017/9/3
Y1 - 2017/9/3
N2 - Many relict and endemic legumes are often rare and endangered species, located in remote and almost impenetrable areas. This makes it difficult to study their symbiosis with nodule bacteria. The aim of this work was to obtain root nodules under laboratory conditions of Hedysarum zundukii, Oxytropis popoviana, Oxytropis triphylla, Oxytropis tragacanthoides, Astragalus chorinensis and Glycyrrhiza uralensis belonging to the Miocene-Pliocene relicts, usually growing in restricted habitats of the Baikal Lake region. A pot experiment was conducted with soils and seeds collected in distribution areas of these legumes. The collected soils had low content of N and P, suggesting importance of nitrogen-fixing symbiosis for successful maintenance of plant populations. The symbiotic nodules were observed on roots of all plant species tested and 52 bacterial strains assumed as root nodule bacteria (rhizobia) were isolated from nodules. The proposed method avoids collection of native plants and prevents decrease in plant populations. The isolated bacteria will be used to search for ancestral genes responsible for the specificity and efficiency of rhizobia-legume symbiosis and can contribute to the conservation of rare and endangered species of relict legumes in the Baikal region.
AB - Many relict and endemic legumes are often rare and endangered species, located in remote and almost impenetrable areas. This makes it difficult to study their symbiosis with nodule bacteria. The aim of this work was to obtain root nodules under laboratory conditions of Hedysarum zundukii, Oxytropis popoviana, Oxytropis triphylla, Oxytropis tragacanthoides, Astragalus chorinensis and Glycyrrhiza uralensis belonging to the Miocene-Pliocene relicts, usually growing in restricted habitats of the Baikal Lake region. A pot experiment was conducted with soils and seeds collected in distribution areas of these legumes. The collected soils had low content of N and P, suggesting importance of nitrogen-fixing symbiosis for successful maintenance of plant populations. The symbiotic nodules were observed on roots of all plant species tested and 52 bacterial strains assumed as root nodule bacteria (rhizobia) were isolated from nodules. The proposed method avoids collection of native plants and prevents decrease in plant populations. The isolated bacteria will be used to search for ancestral genes responsible for the specificity and efficiency of rhizobia-legume symbiosis and can contribute to the conservation of rare and endangered species of relict legumes in the Baikal region.
KW - Baikal relict legumes
KW - root nodule bacteria
UR - http://www.scopus.com/inward/record.url?scp=85028597261&partnerID=8YFLogxK
U2 - 10.1080/00207233.2017.1283948
DO - 10.1080/00207233.2017.1283948
M3 - Article
AN - SCOPUS:85028597261
VL - 74
SP - 700
EP - 705
JO - International Journal of Environmental Studies
JF - International Journal of Environmental Studies
SN - 0020-7233
IS - 5
ER -
ID: 11204941