TY - JOUR

T1 - L-tyrosine-stabilized silver nanoparticles for colorimetric detection of hydrogen peroxide

AU - Сыч, Томаш Сергеевич

AU - Шеховцов, Николай Викторович

AU - Буглак, Андрей Андреевич

AU - Кононов, Алексей Игоревич

PY - 2026/6/5

Y1 - 2026/6/5

N2 - Hydrogen peroxide (H2O2) is a type of reactive oxygen species widespread in biological tissues. It is an important biomarker of oxidative stress, cancers, asthma, etc. In this regard, development of precise and cost-effective sensors for detection of H2O2 in vitro and in vivo is of great significance for biomedicine. In this paper, a colorimetric method is introduced for sensing hydrogen peroxide using tyrosine-stabilized silver nanoparticles. In aqueous solutions, the colorimetric method demonstrated a linear range of 0.4–5 μM with a detection limit (LOD) of 150 nM, whereas in human blood serum the linear range was within 0.7–20 μM characterized by a LOD of 250 nM. Recovery tests in blood serum yielded results within the acceptable range 96–101%, confirming the method's accuracy and reliability. Possible mechanisms of silver nanoparticles/nanoclusters interaction with H2O2 during its detection were examined using fluorescence spectroscopy and dynamic light scattering methods.

AB - Hydrogen peroxide (H2O2) is a type of reactive oxygen species widespread in biological tissues. It is an important biomarker of oxidative stress, cancers, asthma, etc. In this regard, development of precise and cost-effective sensors for detection of H2O2 in vitro and in vivo is of great significance for biomedicine. In this paper, a colorimetric method is introduced for sensing hydrogen peroxide using tyrosine-stabilized silver nanoparticles. In aqueous solutions, the colorimetric method demonstrated a linear range of 0.4–5 μM with a detection limit (LOD) of 150 nM, whereas in human blood serum the linear range was within 0.7–20 μM characterized by a LOD of 250 nM. Recovery tests in blood serum yielded results within the acceptable range 96–101%, confirming the method's accuracy and reliability. Possible mechanisms of silver nanoparticles/nanoclusters interaction with H2O2 during its detection were examined using fluorescence spectroscopy and dynamic light scattering methods.

KW - Blood serum

KW - Colorimetric detection

KW - Dynamic light scattering

KW - Hydrogen peroxide

KW - Silver nanoparticles

UR - https://www.mendeley.com/catalogue/ea23be5d-7659-3c87-9471-0bd03d217a15/

U2 - 10.1016/j.saa.2026.127631

DO - 10.1016/j.saa.2026.127631

M3 - Article

VL - 354

JO - SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

JF - SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

SN - 1386-1425

M1 - 127631

ER -