Research output: Contribution to journal › Article › peer-review
Intranasal epitalon infusion modulates neuronal activity in the rat neocortex. / Sibarov, D. A.; Vol'nova, A. B.; Frolov, D. S.; Nosdrachev, A. D.
In: Rossiǐskii fiziologicheskiǐ zhurnal imeni I.M. Sechenova / Rossiǐskaia akademiia nauk, Vol. 92, No. 8, 08.2006, p. 949-956.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Intranasal epitalon infusion modulates neuronal activity in the rat neocortex
AU - Sibarov, D. A.
AU - Vol'nova, A. B.
AU - Frolov, D. S.
AU - Nosdrachev, A. D.
PY - 2006/8
Y1 - 2006/8
N2 - Properties of tetrapeptide epitalon (Ala-Glu-Asp-Gly) constructed on the basis of pineal peptide extract, have been studied. The intranasal infusions: a noninvasive way to deliver this peptide to CNS hypassing the blood-brain barrier, was used. The aim of the study is to estimate epitalon action on rat motor cortex spontaneous activity. Wistar male rats were anesthetized with urethane (1 g/kg). Extracellular unit recording was made using glass microelectrodes (1-2 MOhm). After recording of spontaneous activity (10-15 min), epitalon intranasal infusion (2 ng) was followed by 30-minute recording. Within a few minutes after the infusion, significant activation of neural activity was observed (2-2.5-fold higher frequency of neuronal spikes). Complex response consisting of several phases was identified in some recordings. The spikes frequency growth during 5 to 7 min (first phase) after the infusion was followed by the second (11-12 min) and the third (17-18 min) phases. An increase of neuronal spontaneous activity was conditioned by the higher frequency of already active units and by the involvement of previously silent cells. At least the first phase of epitalon action can be explained by direct action of the peptide on the cells of the motor cortex.
AB - Properties of tetrapeptide epitalon (Ala-Glu-Asp-Gly) constructed on the basis of pineal peptide extract, have been studied. The intranasal infusions: a noninvasive way to deliver this peptide to CNS hypassing the blood-brain barrier, was used. The aim of the study is to estimate epitalon action on rat motor cortex spontaneous activity. Wistar male rats were anesthetized with urethane (1 g/kg). Extracellular unit recording was made using glass microelectrodes (1-2 MOhm). After recording of spontaneous activity (10-15 min), epitalon intranasal infusion (2 ng) was followed by 30-minute recording. Within a few minutes after the infusion, significant activation of neural activity was observed (2-2.5-fold higher frequency of neuronal spikes). Complex response consisting of several phases was identified in some recordings. The spikes frequency growth during 5 to 7 min (first phase) after the infusion was followed by the second (11-12 min) and the third (17-18 min) phases. An increase of neuronal spontaneous activity was conditioned by the higher frequency of already active units and by the involvement of previously silent cells. At least the first phase of epitalon action can be explained by direct action of the peptide on the cells of the motor cortex.
UR - http://www.scopus.com/inward/record.url?scp=33846903370&partnerID=8YFLogxK
M3 - статья
C2 - 17217245
AN - SCOPUS:33846903370
VL - 92
SP - 949
EP - 956
JO - РОССИЙСКИЙ ФИЗИОЛОГИЧЕСКИЙ ЖУРНАЛ ИМ. И.М. СЕЧЕНОВА
JF - РОССИЙСКИЙ ФИЗИОЛОГИЧЕСКИЙ ЖУРНАЛ ИМ. И.М. СЕЧЕНОВА
SN - 0869-8139
IS - 8
ER -
ID: 9701967