Introduction: Skin diseases such as atopic dermatitis (AD) depends on impaired immunological and microbial balance of microbiota.
The aim: Comparative analysis of microbiota structure of normal human skin and with AD.
Materials and methods: Skin samples of 107 patients with AD and control samples of the skin of healthy human were examined. Bacterial biofilms (BB) were detected on transmission electron microscope JEM-100C (JEOL, Japan).
Results: A comparative electron microscopic analysis of the corneous layer of the epidermis revealed a difference of morphological properties and physiological activity of bacteria in BB in normal and AD skin. In a healthy skin physiologically inactive (resting) and lysed bacteria prevailed. BB in AD skin contained a much larger number, than in control, physiologically active and dividing cells.
Conclusion: To suppress the growth of physiologically active cells inside BB in corneous layer it is necessary to destroy the complex of protective structures (surface film, intercellular matrix) that prevent the exposure of antibacterial drugs and immune protection factors.
Our preliminary experiments have shown destructive effect on the protective structures of BB has a helium low-temperature plasma and bacteriocin-like inhibitory substance of probiotic bacteria.
This work was supported by St. Petersburg State University grant No. 037.218.2016