TY - JOUR

T1 - Effect of Methionine on Gene Expression in Komagataella phaffii Cells

AU - Яньшина, Татьяна Михайловна

AU - Сидорин, Антон Витальевич

AU - Петрова, Кристина Дмитриевна

AU - Шуберт, Мария Андреевна

AU - Макеева, Анастасия Станиславовна

AU - Самбук, Елена Викторовна

AU - Говди, Анастасия Иосифовна

AU - Румянцев, Андрей Михайлович

AU - Падкина, Марина Владимировна

PY - 2023/3/29

Y1 - 2023/3/29

N2 - Komagataella phaffii yeast plays a prominent role in modern biotechnology as a recombinant protein producer. For efficient use of this yeast, it is essential to study the effects of different media components on its growth and gene expression. We investigated the effect of methionine on gene expression in K. phaffii cells using RNA-seq analysis. Several gene groups exhibited altered expression when K. phaffii cells were cultured in a medium with methanol and methionine, compared to a medium without this amino acid. Methionine primarily affects the expression of genes involved in its biosynthesis, fatty acid metabolism, and methanol utilization. The AOX1 gene promoter, which is widely used for heterologous expression in K. phaffii, is downregulated in methionine-containing media. Despite great progress in the development of K. phaffii strain engineering techniques, a sensitive adjustment of cultivation conditions is required to achieve a high yield of the target product. The revealed effect of methionine on K. phaffii gene expression is important for optimizing media recipes and cultivation strategies aimed at maximizing the efficiency of recombinant product synthesis.

AB - Komagataella phaffii yeast plays a prominent role in modern biotechnology as a recombinant protein producer. For efficient use of this yeast, it is essential to study the effects of different media components on its growth and gene expression. We investigated the effect of methionine on gene expression in K. phaffii cells using RNA-seq analysis. Several gene groups exhibited altered expression when K. phaffii cells were cultured in a medium with methanol and methionine, compared to a medium without this amino acid. Methionine primarily affects the expression of genes involved in its biosynthesis, fatty acid metabolism, and methanol utilization. The AOX1 gene promoter, which is widely used for heterologous expression in K. phaffii, is downregulated in methionine-containing media. Despite great progress in the development of K. phaffii strain engineering techniques, a sensitive adjustment of cultivation conditions is required to achieve a high yield of the target product. The revealed effect of methionine on K. phaffii gene expression is important for optimizing media recipes and cultivation strategies aimed at maximizing the efficiency of recombinant product synthesis.

KW - AOX1 promoter

KW - Komagataella phaffii

KW - Pichia pastoris

KW - X-33

KW - amino acid metabolism

KW - fatty acid metabolism

KW - methanol metabolism

KW - methionine

UR - https://www.mendeley.com/catalogue/6c56a4af-9d95-3c7f-999a-f26ed154ac9f/

U2 - 10.3390/microorganisms11040877

DO - 10.3390/microorganisms11040877

M3 - Article

C2 - 37110303

VL - 11

JO - Microorganisms

JF - Microorganisms

SN - 2076-2607

IS - 4

M1 - 877

ER -