Distinct pools of synaptic vesicles in neurotransmitter release. / Pieribone, Vincent A.; Shupliakov, Oleg; Brodin, Lennart; Hilfiker-Rothenfluh, Sabine; Czernik, Andrew J.; Greengard, Paul.
In: Nature, Vol. 375, No. 6531, 08.06.1995, p. 493-497.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Distinct pools of synaptic vesicles in neurotransmitter release
AU - Pieribone, Vincent A.
AU - Shupliakov, Oleg
AU - Brodin, Lennart
AU - Hilfiker-Rothenfluh, Sabine
AU - Czernik, Andrew J.
AU - Greengard, Paul
PY - 1995/6/8
Y1 - 1995/6/8
N2 - NERVE terminals are unique among cellular secretory systems in that they can sustain vesicular release at a high rate. Although little is known about the mechanisms that account for the distinctive features of neurotransmitter release, it can be assumed that neuron-specific proteins are involved. One such protein family, the synapsins, are believed to regulate neurotransmitter release through phosphorylation-dependent interactions with synaptic vesicles and cytoskeletal elements1. Here we show that clusters of vesicles at synaptic release sites are composed of two pools, a distal pool containing synapsin and a proximal pool devoid of synapsin and located adjacent to the presynaptic membrane. Pre-synaptic injection of synapsin antibodies resulted in the loss of the distal pool, without any apparent effect on the proximal pool. Depletion of this distal pool was associated with a marked depression of neurotransmitter release evoked by high-frequency (18-20 Hz) but not by low-frequency (0.2 Hz) stimulation. Thus the availability of the synapsin-associated pool of vesicles seems to be required to sustain release of neurotransmitter in response to high-frequency bursts of impulses.
AB - NERVE terminals are unique among cellular secretory systems in that they can sustain vesicular release at a high rate. Although little is known about the mechanisms that account for the distinctive features of neurotransmitter release, it can be assumed that neuron-specific proteins are involved. One such protein family, the synapsins, are believed to regulate neurotransmitter release through phosphorylation-dependent interactions with synaptic vesicles and cytoskeletal elements1. Here we show that clusters of vesicles at synaptic release sites are composed of two pools, a distal pool containing synapsin and a proximal pool devoid of synapsin and located adjacent to the presynaptic membrane. Pre-synaptic injection of synapsin antibodies resulted in the loss of the distal pool, without any apparent effect on the proximal pool. Depletion of this distal pool was associated with a marked depression of neurotransmitter release evoked by high-frequency (18-20 Hz) but not by low-frequency (0.2 Hz) stimulation. Thus the availability of the synapsin-associated pool of vesicles seems to be required to sustain release of neurotransmitter in response to high-frequency bursts of impulses.
UR - http://www.scopus.com/inward/record.url?scp=0029054397&partnerID=8YFLogxK
U2 - 10.1038/375493a0
DO - 10.1038/375493a0
M3 - Article
C2 - 7777058
AN - SCOPUS:0029054397
VL - 375
SP - 493
EP - 497
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6531
ER -
ID: 40837167