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Curcumin by activation of adenosine A2A receptor stimulates protein kinase a and potentiates inhibitory effect of cangrelor on platelets. / Rukoyatkina, Natalia; Shpakova, Valentina; Bogoutdinova, Alina; Kharazova, Alexandra; Mindukshev, Igor; Gambaryan, Stepan.

In: Biochemical and Biophysical Research Communications, Vol. 586, 01.01.2022, p. 20-26.

Research output: Contribution to journal › Article › peer-review

Harvard

Rukoyatkina, N, Shpakova, V, Bogoutdinova, A, Kharazova, A, Mindukshev, I & Gambaryan, S 2022, 'Curcumin by activation of adenosine A2A receptor stimulates protein kinase a and potentiates inhibitory effect of cangrelor on platelets', Biochemical and Biophysical Research Communications, vol. 586, pp. 20-26. https://doi.org/10.1016/j.bbrc.2021.11.006

APA

Rukoyatkina, N., Shpakova, V., Bogoutdinova, A., Kharazova, A., Mindukshev, I., & Gambaryan, S. (2022). Curcumin by activation of adenosine A2A receptor stimulates protein kinase a and potentiates inhibitory effect of cangrelor on platelets. Biochemical and Biophysical Research Communications, 586, 20-26. https://doi.org/10.1016/j.bbrc.2021.11.006

Vancouver

Rukoyatkina N, Shpakova V, Bogoutdinova A, Kharazova A, Mindukshev I, Gambaryan S. Curcumin by activation of adenosine A2A receptor stimulates protein kinase a and potentiates inhibitory effect of cangrelor on platelets. Biochemical and Biophysical Research Communications. 2022 Jan 1;586:20-26. https://doi.org/10.1016/j.bbrc.2021.11.006

Author

Rukoyatkina, Natalia ; Shpakova, Valentina ; Bogoutdinova, Alina ; Kharazova, Alexandra ; Mindukshev, Igor ; Gambaryan, Stepan. / Curcumin by activation of adenosine A2A receptor stimulates protein kinase a and potentiates inhibitory effect of cangrelor on platelets. In: Biochemical and Biophysical Research Communications. 2022 ; Vol. 586. pp. 20-26.

BibTeX

@article{5857597687da4168afbc567eb3a9abe0,
title = "Curcumin by activation of adenosine A2A receptor stimulates protein kinase a and potentiates inhibitory effect of cangrelor on platelets",
abstract = "Curcumin is a natural polyphenol derived from the turmeric plant (Curcuma longa) which exhibits numerous beneficial effects on different cell types. Inhibition of platelet activation by curcumin is well known, however molecular mechanisms of its action on platelets are not fully defined. In this study, we used laser diffraction method for analysis of platelet aggregation and Western blot for analysis of intracellular signaling mechanisms of curcumin effects on platelets. We identified two new molecular mechanisms involved in the inhibitory effects of curcumin on platelet activation. Firstly, curcumin by activation of adenosine A2A receptor stimulated protein kinase A activation and phosphorylation of Vasodilator-stimulated phosphoprotein. Secondly, we demonstrated that curcumin even at low doses, which did not inhibit platelet aggregation, potentiated inhibitory effect of ADP receptor P2Y12 antagonist cangrelor which partly could be explained by activation of adenosine A2A receptor.",
keywords = "A receptor, Adenosine, Curcumin, Platelets, Protein kinase A, Phosphorylation, Humans, Receptor, Adenosine A2A/genetics, Adenosine Monophosphate/analogs & derivatives, Purinergic P2Y Receptor Antagonists/pharmacology, Curcumin/isolation & purification, Plant Extracts/chemistry, Microfilament Proteins/genetics, Platelet Aggregation Inhibitors/pharmacology, Signal Transduction, Blood Platelets/cytology, Gene Expression Regulation, Cyclic AMP-Dependent Protein Kinases/genetics, Platelet Activation/drug effects, Drug Synergism, Phosphoproteins/genetics, Receptors, Purinergic P2Y12/genetics, Cell Adhesion Molecules/genetics, Primary Cell Culture, Curcuma/chemistry, Adenosine Diphosphate/pharmacology, A(2A) receptor, INJURY, LONGA, CLOPIDOGREL, PATHWAY, NITRIC-OXIDE, ALTERS, CELLS",
author = "Natalia Rukoyatkina and Valentina Shpakova and Alina Bogoutdinova and Alexandra Kharazova and Igor Mindukshev and Stepan Gambaryan",
note = "Publisher Copyright: {\textcopyright} 2021",
year = "2022",
month = jan,
day = "1",
doi = "10.1016/j.bbrc.2021.11.006",
language = "English",
volume = "586",
pages = "20--26",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Curcumin by activation of adenosine A2A receptor stimulates protein kinase a and potentiates inhibitory effect of cangrelor on platelets

AU - Rukoyatkina, Natalia

AU - Shpakova, Valentina

AU - Bogoutdinova, Alina

AU - Kharazova, Alexandra

AU - Mindukshev, Igor

AU - Gambaryan, Stepan

N1 - Publisher Copyright: © 2021

PY - 2022/1/1

Y1 - 2022/1/1

N2 - Curcumin is a natural polyphenol derived from the turmeric plant (Curcuma longa) which exhibits numerous beneficial effects on different cell types. Inhibition of platelet activation by curcumin is well known, however molecular mechanisms of its action on platelets are not fully defined. In this study, we used laser diffraction method for analysis of platelet aggregation and Western blot for analysis of intracellular signaling mechanisms of curcumin effects on platelets. We identified two new molecular mechanisms involved in the inhibitory effects of curcumin on platelet activation. Firstly, curcumin by activation of adenosine A2A receptor stimulated protein kinase A activation and phosphorylation of Vasodilator-stimulated phosphoprotein. Secondly, we demonstrated that curcumin even at low doses, which did not inhibit platelet aggregation, potentiated inhibitory effect of ADP receptor P2Y12 antagonist cangrelor which partly could be explained by activation of adenosine A2A receptor.

AB - Curcumin is a natural polyphenol derived from the turmeric plant (Curcuma longa) which exhibits numerous beneficial effects on different cell types. Inhibition of platelet activation by curcumin is well known, however molecular mechanisms of its action on platelets are not fully defined. In this study, we used laser diffraction method for analysis of platelet aggregation and Western blot for analysis of intracellular signaling mechanisms of curcumin effects on platelets. We identified two new molecular mechanisms involved in the inhibitory effects of curcumin on platelet activation. Firstly, curcumin by activation of adenosine A2A receptor stimulated protein kinase A activation and phosphorylation of Vasodilator-stimulated phosphoprotein. Secondly, we demonstrated that curcumin even at low doses, which did not inhibit platelet aggregation, potentiated inhibitory effect of ADP receptor P2Y12 antagonist cangrelor which partly could be explained by activation of adenosine A2A receptor.

KW - A receptor

KW - Adenosine

KW - Curcumin

KW - Platelets

KW - Protein kinase A

KW - Phosphorylation

KW - Humans

KW - Receptor, Adenosine A2A/genetics

KW - Adenosine Monophosphate/analogs & derivatives

KW - Purinergic P2Y Receptor Antagonists/pharmacology

KW - Curcumin/isolation & purification

KW - Plant Extracts/chemistry

KW - Microfilament Proteins/genetics

KW - Platelet Aggregation Inhibitors/pharmacology

KW - Signal Transduction

KW - Blood Platelets/cytology

KW - Gene Expression Regulation

KW - Cyclic AMP-Dependent Protein Kinases/genetics

KW - Platelet Activation/drug effects

KW - Drug Synergism

KW - Phosphoproteins/genetics

KW - Receptors, Purinergic P2Y12/genetics

KW - Cell Adhesion Molecules/genetics

KW - Primary Cell Culture

KW - Curcuma/chemistry

KW - Adenosine Diphosphate/pharmacology

KW - A(2A) receptor

KW - INJURY

KW - LONGA

KW - CLOPIDOGREL

KW - PATHWAY

KW - NITRIC-OXIDE

KW - ALTERS

KW - CELLS

UR - http://www.scopus.com/inward/record.url?scp=85119400847&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2021.11.006

DO - 10.1016/j.bbrc.2021.11.006

M3 - Article

C2 - 34823218

AN - SCOPUS:85119400847

VL - 586

SP - 20

EP - 26

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

ER -

ID: 92256946