The study presents cryoscopic investigation of binary systems containing adducts of fullerene C60 with sulfur-containing amino acids C60(C3H7NO2S)3 (adduct with L-cysteine, C60-Cys) and C60(C5H11NO2S)3 (adduct with L-methionine, C60-Met). Excess thermodynamic functions of solution components were calculated using a semi-empirical model Virial Decomposition Asymmetric Model (VD-AS). Adducts of fullerene C60 with sulfur-containing amino acids were characterized using 13C NMR, IR and UV/Vis spectroscopy, elemental analysis, and HPLC. Additionally, experimental data on the enthalpy of combustion of fullerene adducts, as well as concentration dependences of particle size distribution and ζ-potentials are presented.