In this study, a novel catalyst based on hydroxyapatite doped by cobalt for chemiluminescence reaction of luminol oxidation by H₂O₂ was suggested for the first time. The catalyst nanoparticles were synthesized by a hydrothermal method and characterized by various methods including density functional theory calculations. The impact of nanoparticles sizes, surface composition, contact efficiency and crystallinity on chemiluminescence intensity were investigated. The maximum chemiluminescence intensity was obtained for polycrystalline nanoparticles. This phenomenon was studied in detail and applied for chemiluminescence analysis for the first time. The chemiluminescence determination of sulfonamides as model analytes was considered. The sensing was based on sulfonamides-dependent quenching of the chemiluminescence intensity in the presence of novel catalyst existed as an aqueous suspension.