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Adsorbed plasma proteins modulate the effects of single-walled carbon nanotubes on neutrophils in blood. / Vlasova, I.I.; Mikhalchik, E.V.; Barinov, N.A.; Kostevich, V.A.; Smolina, N.V.; Klinov, D.V.; Sokolov, A.V.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 12, No. 6, 2016, p. 1615–1625.

Research output: Contribution to journal › Article

Harvard

Vlasova, II, Mikhalchik, EV, Barinov, NA, Kostevich, VA, Smolina, NV, Klinov, DV & Sokolov, AV 2016, 'Adsorbed plasma proteins modulate the effects of single-walled carbon nanotubes on neutrophils in blood', Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 12, no. 6, pp. 1615–1625. https://doi.org/10.1016/j.nano.2016.02.012

APA

Vlasova, I. I., Mikhalchik, E. V., Barinov, N. A., Kostevich, V. A., Smolina, N. V., Klinov, D. V., & Sokolov, A. V. (2016). Adsorbed plasma proteins modulate the effects of single-walled carbon nanotubes on neutrophils in blood. Nanomedicine: Nanotechnology, Biology, and Medicine, 12(6), 1615–1625. https://doi.org/10.1016/j.nano.2016.02.012

Vancouver

Vlasova II, Mikhalchik EV, Barinov NA, Kostevich VA, Smolina NV, Klinov DV et al. Adsorbed plasma proteins modulate the effects of single-walled carbon nanotubes on neutrophils in blood. Nanomedicine: Nanotechnology, Biology, and Medicine. 2016;12(6):1615–1625. https://doi.org/10.1016/j.nano.2016.02.012

Author

Vlasova, I.I. ; Mikhalchik, E.V. ; Barinov, N.A. ; Kostevich, V.A. ; Smolina, N.V. ; Klinov, D.V. ; Sokolov, A.V. / Adsorbed plasma proteins modulate the effects of single-walled carbon nanotubes on neutrophils in blood. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2016 ; Vol. 12, No. 6. pp. 1615–1625.

BibTeX

@article{4340a2132615445981cc0508bbb9bc92,
title = "Adsorbed plasma proteins modulate the effects of single-walled carbon nanotubes on neutrophils in blood",
abstract = "Proteins adsorbed on a surface may affect the interaction of this surface with cells. Here, we studied the binding of human serum albumin (HSA), fibrinogen (FBG) and immunoglobulin G (IgG) to PEGylated single-walled carbon nanotubes (PEG-SWCNTs) and evaluated the impact of PEG-SWCNT treated by these proteins on neutrophils in whole blood samples. Measurements of adsorption parameters revealed tight binding of proteins to PEG-SWCNTs. AFM was employed to directly observe protein binding to side walls of PEG-SWCNTs. Fluorescein-labeled IgG was used to ascertain the stability of PEG-SWCNT-IgG complexes in plasma. In blood samples, all plasma proteins mitigated damage of neutrophils observed just after blood exposure to PEG-SWCNTs, while only treatment of PEG-SWCNTs with IgG resulted in dose- and time-dependent enhancement of CNT-induced neutrophil activation and in potentiation of oxidative stress. Our study demonstrates the ability of adsorbed plasma proteins to influence neutrophil response caused by PEG-SWCNTs",
keywords = "Albumin, Fibrinogen, Immunoglobulin G, Myeloperoxidase, Neutrophil activation, PEGylated carbon nanotubes",
author = "I.I. Vlasova and E.V. Mikhalchik and N.A. Barinov and V.A. Kostevich and N.V. Smolina and D.V. Klinov and A.V. Sokolov",
year = "2016",
doi = "10.1016/j.nano.2016.02.012",
language = "English",
volume = "12",
pages = "1615–1625",
journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",
issn = "1549-9634",
publisher = "Elsevier",
number = "6",

}

RIS

TY - JOUR

T1 - Adsorbed plasma proteins modulate the effects of single-walled carbon nanotubes on neutrophils in blood

AU - Vlasova, I.I.

AU - Mikhalchik, E.V.

AU - Barinov, N.A.

AU - Kostevich, V.A.

AU - Smolina, N.V.

AU - Klinov, D.V.

AU - Sokolov, A.V.

PY - 2016

Y1 - 2016

N2 - Proteins adsorbed on a surface may affect the interaction of this surface with cells. Here, we studied the binding of human serum albumin (HSA), fibrinogen (FBG) and immunoglobulin G (IgG) to PEGylated single-walled carbon nanotubes (PEG-SWCNTs) and evaluated the impact of PEG-SWCNT treated by these proteins on neutrophils in whole blood samples. Measurements of adsorption parameters revealed tight binding of proteins to PEG-SWCNTs. AFM was employed to directly observe protein binding to side walls of PEG-SWCNTs. Fluorescein-labeled IgG was used to ascertain the stability of PEG-SWCNT-IgG complexes in plasma. In blood samples, all plasma proteins mitigated damage of neutrophils observed just after blood exposure to PEG-SWCNTs, while only treatment of PEG-SWCNTs with IgG resulted in dose- and time-dependent enhancement of CNT-induced neutrophil activation and in potentiation of oxidative stress. Our study demonstrates the ability of adsorbed plasma proteins to influence neutrophil response caused by PEG-SWCNTs

AB - Proteins adsorbed on a surface may affect the interaction of this surface with cells. Here, we studied the binding of human serum albumin (HSA), fibrinogen (FBG) and immunoglobulin G (IgG) to PEGylated single-walled carbon nanotubes (PEG-SWCNTs) and evaluated the impact of PEG-SWCNT treated by these proteins on neutrophils in whole blood samples. Measurements of adsorption parameters revealed tight binding of proteins to PEG-SWCNTs. AFM was employed to directly observe protein binding to side walls of PEG-SWCNTs. Fluorescein-labeled IgG was used to ascertain the stability of PEG-SWCNT-IgG complexes in plasma. In blood samples, all plasma proteins mitigated damage of neutrophils observed just after blood exposure to PEG-SWCNTs, while only treatment of PEG-SWCNTs with IgG resulted in dose- and time-dependent enhancement of CNT-induced neutrophil activation and in potentiation of oxidative stress. Our study demonstrates the ability of adsorbed plasma proteins to influence neutrophil response caused by PEG-SWCNTs

KW - Albumin

KW - Fibrinogen

KW - Immunoglobulin G

KW - Myeloperoxidase

KW - Neutrophil activation

KW - PEGylated carbon nanotubes

U2 - 10.1016/j.nano.2016.02.012

DO - 10.1016/j.nano.2016.02.012

M3 - Article

VL - 12

SP - 1615

EP - 1625

JO - Nanomedicine: Nanotechnology, Biology, and Medicine

JF - Nanomedicine: Nanotechnology, Biology, and Medicine

SN - 1549-9634

IS - 6

ER -

ID: 7564580