• I.I. Vlasova
  • E.V. Mikhalchik
  • N.A. Barinov
  • V.A. Kostevich
  • N.V. Smolina
  • D.V. Klinov
  • A.V. Sokolov
Proteins adsorbed on a surface may affect the interaction of this surface with cells. Here, we studied the binding of human serum albumin (HSA), fibrinogen (FBG) and immunoglobulin G (IgG) to PEGylated single-walled carbon nanotubes (PEG-SWCNTs) and evaluated the impact of PEG-SWCNT treated by these proteins on neutrophils in whole blood samples. Measurements of adsorption parameters revealed tight binding of proteins to PEG-SWCNTs. AFM was employed to directly observe protein binding to side walls of PEG-SWCNTs. Fluorescein-labeled IgG was used to ascertain the stability of PEG-SWCNT-IgG complexes in plasma. In blood samples, all plasma proteins mitigated damage of neutrophils observed just after blood exposure to PEG-SWCNTs, while only treatment of PEG-SWCNTs with IgG resulted in dose- and time-dependent enhancement of CNT-induced neutrophil activation and in potentiation of oxidative stress. Our study demonstrates the ability of adsorbed plasma proteins to influence neutrophil response caused by PEG-SWCNTs
Original languageEnglish
Pages (from-to)1615–1625
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume12
Issue number6
DOIs
StatePublished - 2016
Externally publishedYes

    Research areas

  • Albumin, Fibrinogen, Immunoglobulin G, Myeloperoxidase, Neutrophil activation, PEGylated carbon nanotubes

ID: 7564580