Research output: Contribution to journal › Article › peer-review
СИГНАЛ ЯДЕРНОЙ ЛОКАЛИЗАЦИИ ГАММА-ИНТЕРФЕРОНА БЫКА ОБЕСПЕЧИВАЕТ ТРАНСЛОКАЦИЮ РЕКОМБИНАНТНОГО БЕЛКА В ЯДРО КЛЕТКИ ДРОЖЖЕЙ PICHIA PASTORIS. / Gradoboeva, A. E.; Padkina, M. V.
In: Цитология, Vol. 52, No. 10, 2010, p. 863-868.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - СИГНАЛ ЯДЕРНОЙ ЛОКАЛИЗАЦИИ ГАММА-ИНТЕРФЕРОНА БЫКА ОБЕСПЕЧИВАЕТ ТРАНСЛОКАЦИЮ РЕКОМБИНАНТНОГО БЕЛКА В ЯДРО КЛЕТКИ ДРОЖЖЕЙ PICHIA PASTORIS
AU - Gradoboeva, A. E.
AU - Padkina, M. V.
PY - 2010
Y1 - 2010
N2 - It is known that heterologous protein production in microorganisms has negative influence upon a host cell. In this regard, of particular interest is to know the fate of recombinant proteins in a cell. In this study, it was shown that NLS of bovine gamma-interferon functions in yeast Pichia pastoris cells. The absence of the C-terminal NLS of bovine gamma-interferon leads to cytoplasmic localization of the recombinant protein.
AB - It is known that heterologous protein production in microorganisms has negative influence upon a host cell. In this regard, of particular interest is to know the fate of recombinant proteins in a cell. In this study, it was shown that NLS of bovine gamma-interferon functions in yeast Pichia pastoris cells. The absence of the C-terminal NLS of bovine gamma-interferon leads to cytoplasmic localization of the recombinant protein.
KW - Bovine gamma-interferon
KW - Nuclear localization sequence
KW - Recombinant proteins
UR - http://www.scopus.com/inward/record.url?scp=79952257362&partnerID=8YFLogxK
M3 - статья
C2 - 21268843
VL - 52
SP - 863
EP - 868
JO - Цитология
JF - Цитология
SN - 0041-3771
IS - 10
ER -
ID: 5120583