The colon has pronounced barrier properties. Inflammatory diseases and colon cancer are associated with a decrease in these properties. At the same time, the incidence of the intestine in pathologies, as well as its barrier properties, are not the same — in colorectal cancer, tumors are more often formed in the distal part of the intestine and are much more rare in its proximal part. Also, bowel diseases are accompanied by an increased content of cytokines and eicosanoids secreted by the intestinal wall, endothelium and associated immune cells. The main pro-inflammatory cytokines that promote the progression of bowel diseases are TNFα and IL-6, while IL-10, on the contrary, has antiinflammatory properties. The aim of our work was to investigate the barrier properties of the proximal and distal parts of the rat colon under the action of TNFα, IL-6 and IL-10 from the apical side of the epithelium. Cytokine exposure was performed both in vivo and ex vivo. Electrophysiological parameters were investigated — transepithelial resistance and short-circuit current, as well as paracellular permeability for sodium fluorescein solution. According to the study, TNFα weakened the barrier properties of the distal colon. It has increased short-circuit current and paracellular permeability. IL-6 also increased these values in the distal region and decreased transepithelial resistance. IL-10 influenced the proximal segments, increasing paracellular permeability and decreasing transepithelial resistance. Thus, TNFα, IL-6 and IL-10, acting from the apical side of epithelial cells, increased the permeability of the colon. However, the effect of each cytokine was unique. We believe that the regulatory effects of the studied cytokines can cause impairment of the barrier properties of the colon and different morbidity of its departments in pathologies.