СРАВНИТЕЛЬНЫЙ АНАЛИЗ ЭКСПРЕССИИ МОЛЕКУЛЯРНЫХ МАРКЕРОВ ДИСФУНКЦИИ ПЕРВИЧНЫХ ЭНДОТЕЛИАЛЬНЫХ КЛЕТОК КОРОНАРНОЙ И ВНУТРЕННЕЙ ГРУДНОЙ АРТЕРИИ ЧЕЛОВЕКА

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СРАВНИТЕЛЬНЫЙ АНАЛИЗ ЭКСПРЕССИИ МОЛЕКУЛЯРНЫХ МАРКЕРОВ ДИСФУНКЦИИ ПЕРВИЧНЫХ ЭНДОТЕЛИАЛЬНЫХ КЛЕТОК КОРОНАРНОЙ И ВНУТРЕННЕЙ ГРУДНОЙ АРТЕРИИ ЧЕЛОВЕКА. / Markova, V.E.; Shishkova, D.K.; Stepanov, A.D.; Frolov, A.V.; Yurieva, Y.O.; Lazebnaya, A.I.; Repkin, E.A.; Kabilov, M.R.; Tupikin, A.E.; Kutikhin, A.G.

In: Комплексные проблемы сердечно-сосудистых заболеваний, Vol. 14, No. 6, 2025, p. 80-104.

Research output: Contribution to journal › Article › peer-review

Author

Markova, V.E. ; Shishkova, D.K. ; Stepanov, A.D. ; Frolov, A.V. ; Yurieva, Y.O. ; Lazebnaya, A.I. ; Repkin, E.A. ; Kabilov, M.R. ; Tupikin, A.E. ; Kutikhin, A.G. / СРАВНИТЕЛЬНЫЙ АНАЛИЗ ЭКСПРЕССИИ МОЛЕКУЛЯРНЫХ МАРКЕРОВ ДИСФУНКЦИИ ПЕРВИЧНЫХ ЭНДОТЕЛИАЛЬНЫХ КЛЕТОК КОРОНАРНОЙ И ВНУТРЕННЕЙ ГРУДНОЙ АРТЕРИИ ЧЕЛОВЕКА. In: Комплексные проблемы сердечно-сосудистых заболеваний. 2025 ; Vol. 14, No. 6. pp. 80-104.

BibTeX

@article{f7214180ac4f4e6ca0a7199a347eb7f1,

title = "СРАВНИТЕЛЬНЫЙ АНАЛИЗ ЭКСПРЕССИИ МОЛЕКУЛЯРНЫХ МАРКЕРОВ ДИСФУНКЦИИ ПЕРВИЧНЫХ ЭНДОТЕЛИАЛЬНЫХ КЛЕТОК КОРОНАРНОЙ И ВНУТРЕННЕЙ ГРУДНОЙ АРТЕРИИ ЧЕЛОВЕКА",

abstract = "Endothelial cell dysfunction is accompanied by reduced expression of genes encoding endothelial phenotype markers, basement membrane proteins, and extracellular matrix components. • Primary human coronary artery and internal thoracic artery endothelial cells have distinct molecularprofile of endothelial phenotype markers, basement membrane components, and angiogenic proteins. • In comparison with primary human coronary artery endothelial cells, profile of basement membrane and extracellular matrix components of human internal thoracic artery endothelial cells is more similarto intact endothelial cells. Aim To compare the gene and protein expression profile in primary human coronary artery endothelial cells (HCAEC) and human internal thoracic artery endothelial cells (HITAEC) in physiological and dysfunctional states. Methods To perform an unbiased and high-throughput analysis, we applied RNA sequencing and ultra-high performance liquid chromatography-tandem massspectrometry. Fold change and transcripts per million were used as metrics to evaluate RNA sequencing results. Mass spectrometry data were assessed by ranking the expression of 2,986 detected molecules after measuring areas of respective chromatographic peaks. Results Dysfunctional ECs showed decreased expression of the genes encoding endothelialphenotype markers, basement membrane components, extracellular matrix proteins, and angiogenic molecules, concurrently having higher expression of the genes encoding oxidative and endoplasmic reticulum stress proteins. In comparison to HITAEC, HCAEC had higher expression of angiogenic proteins (including B-type ephrin receptors EPHB2 and EPHB4) and both subunits of endothelial integrin αvβ3 (ITGAV and ITGB3). In turn, HITAEC had higher expression ofendothelial transcription factors ERG and FLI1, angiopoietins (ANGPT2 and ANGPTL2) and their receptors (PTPRB and TEK), VEGF co-receptors (NRP1 и NRP2), and also higher expression of basement membrane and extracellular matrix components which have been overexpressed in the intact ECs (such as laminin, type IV collagen, von Willebrand factor, angiopoietin-related protein 2, biglycan, nultimerin 1, cathepsin С, and ADAMTS4). Conclusion Dysfunctional ECs have lower expression of basement membrane and extracellularmatrix components. HCAEC and HITAEC have significant differences in the molecular profile of endothelial phenotype markers, basement membrane proteins, extracellular matrix components, and angiogenic proteins. {\textcopyright} 2026, NII KPSSZ. All rights reserved.",

keywords = "Basement membrane, Coronary artery, Endothelial cells, Endothelial dysfunction, Endothelial phenotype markers, Extracellular matrix, Internal thoracic artery, aggrecanase 1, alpha5 integrin, angiogenic protein, angiopoietin 2, angiopoietin related protein 2, beta3 integrin, biglycan, collagen type 4, dipeptidyl peptidase I, ephrin receptor B2, ephrin receptor B4, laminin, molecular marker, nrp1 protein, nrp2 protein, nultimerin 1, ptprb protein, scleroprotein, tek protein, transcription factor ERG, transcription factor Fli 1, unclassified drug, vasculotropin receptor, von Willebrand factor, Article, basement membrane, controlled study, coronary artery, endoplasmic reticulum stress, endothelial dysfunction, extracellular matrix, gene expression profiling, gene overexpression, high throughput sequencing, human, human cell, internal mammary artery, liquid chromatography-mass spectrometry, primary cell, protein expression, RNA sequencing, vascular endothelial cell",

author = "V.E. Markova and D.K. Shishkova and A.D. Stepanov and A.V. Frolov and Y.O. Yurieva and A.I. Lazebnaya and E.A. Repkin and M.R. Kabilov and A.E. Tupikin and A.G. Kutikhin",

note = "Export Date: 09 March 2026; Cited By: 0; Correspondence Address: V.E. Markova; Federal State Budgetary Institution, Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo, 6, blvd. named after academician L.S. Barbarash, ,, ;, 650002, Russian Federation; email: marvika97@gmail.com",

year = "2025",

doi = "10.17802/2306-1278-2025-14-6-80-104",

language = "русский",

volume = "14",

pages = "80--104",

journal = "Комплексные проблемы сердечно-сосудистых заболеваний",

issn = "2306-1278",

publisher = "Научно-исследовательский институт комплексных проблем сердечно-сосудистых заболеваний",

number = "6",

}

RIS

TY - JOUR

T1 - СРАВНИТЕЛЬНЫЙ АНАЛИЗ ЭКСПРЕССИИ МОЛЕКУЛЯРНЫХ МАРКЕРОВ ДИСФУНКЦИИ ПЕРВИЧНЫХ ЭНДОТЕЛИАЛЬНЫХ КЛЕТОК КОРОНАРНОЙ И ВНУТРЕННЕЙ ГРУДНОЙ АРТЕРИИ ЧЕЛОВЕКА

AU - Markova, V.E.

AU - Shishkova, D.K.

AU - Stepanov, A.D.

AU - Frolov, A.V.

AU - Yurieva, Y.O.

AU - Lazebnaya, A.I.

AU - Repkin, E.A.

AU - Kabilov, M.R.

AU - Tupikin, A.E.

AU - Kutikhin, A.G.

N1 - Export Date: 09 March 2026; Cited By: 0; Correspondence Address: V.E. Markova; Federal State Budgetary Institution, Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo, 6, blvd. named after academician L.S. Barbarash, ,, ;, 650002, Russian Federation; email: marvika97@gmail.com

PY - 2025

Y1 - 2025

N2 - Endothelial cell dysfunction is accompanied by reduced expression of genes encoding endothelial phenotype markers, basement membrane proteins, and extracellular matrix components. • Primary human coronary artery and internal thoracic artery endothelial cells have distinct molecularprofile of endothelial phenotype markers, basement membrane components, and angiogenic proteins. • In comparison with primary human coronary artery endothelial cells, profile of basement membrane and extracellular matrix components of human internal thoracic artery endothelial cells is more similarto intact endothelial cells. Aim To compare the gene and protein expression profile in primary human coronary artery endothelial cells (HCAEC) and human internal thoracic artery endothelial cells (HITAEC) in physiological and dysfunctional states. Methods To perform an unbiased and high-throughput analysis, we applied RNA sequencing and ultra-high performance liquid chromatography-tandem massspectrometry. Fold change and transcripts per million were used as metrics to evaluate RNA sequencing results. Mass spectrometry data were assessed by ranking the expression of 2,986 detected molecules after measuring areas of respective chromatographic peaks. Results Dysfunctional ECs showed decreased expression of the genes encoding endothelialphenotype markers, basement membrane components, extracellular matrix proteins, and angiogenic molecules, concurrently having higher expression of the genes encoding oxidative and endoplasmic reticulum stress proteins. In comparison to HITAEC, HCAEC had higher expression of angiogenic proteins (including B-type ephrin receptors EPHB2 and EPHB4) and both subunits of endothelial integrin αvβ3 (ITGAV and ITGB3). In turn, HITAEC had higher expression ofendothelial transcription factors ERG and FLI1, angiopoietins (ANGPT2 and ANGPTL2) and their receptors (PTPRB and TEK), VEGF co-receptors (NRP1 и NRP2), and also higher expression of basement membrane and extracellular matrix components which have been overexpressed in the intact ECs (such as laminin, type IV collagen, von Willebrand factor, angiopoietin-related protein 2, biglycan, nultimerin 1, cathepsin С, and ADAMTS4). Conclusion Dysfunctional ECs have lower expression of basement membrane and extracellularmatrix components. HCAEC and HITAEC have significant differences in the molecular profile of endothelial phenotype markers, basement membrane proteins, extracellular matrix components, and angiogenic proteins. © 2026, NII KPSSZ. All rights reserved.

AB - Endothelial cell dysfunction is accompanied by reduced expression of genes encoding endothelial phenotype markers, basement membrane proteins, and extracellular matrix components. • Primary human coronary artery and internal thoracic artery endothelial cells have distinct molecularprofile of endothelial phenotype markers, basement membrane components, and angiogenic proteins. • In comparison with primary human coronary artery endothelial cells, profile of basement membrane and extracellular matrix components of human internal thoracic artery endothelial cells is more similarto intact endothelial cells. Aim To compare the gene and protein expression profile in primary human coronary artery endothelial cells (HCAEC) and human internal thoracic artery endothelial cells (HITAEC) in physiological and dysfunctional states. Methods To perform an unbiased and high-throughput analysis, we applied RNA sequencing and ultra-high performance liquid chromatography-tandem massspectrometry. Fold change and transcripts per million were used as metrics to evaluate RNA sequencing results. Mass spectrometry data were assessed by ranking the expression of 2,986 detected molecules after measuring areas of respective chromatographic peaks. Results Dysfunctional ECs showed decreased expression of the genes encoding endothelialphenotype markers, basement membrane components, extracellular matrix proteins, and angiogenic molecules, concurrently having higher expression of the genes encoding oxidative and endoplasmic reticulum stress proteins. In comparison to HITAEC, HCAEC had higher expression of angiogenic proteins (including B-type ephrin receptors EPHB2 and EPHB4) and both subunits of endothelial integrin αvβ3 (ITGAV and ITGB3). In turn, HITAEC had higher expression ofendothelial transcription factors ERG and FLI1, angiopoietins (ANGPT2 and ANGPTL2) and their receptors (PTPRB and TEK), VEGF co-receptors (NRP1 и NRP2), and also higher expression of basement membrane and extracellular matrix components which have been overexpressed in the intact ECs (such as laminin, type IV collagen, von Willebrand factor, angiopoietin-related protein 2, biglycan, nultimerin 1, cathepsin С, and ADAMTS4). Conclusion Dysfunctional ECs have lower expression of basement membrane and extracellularmatrix components. HCAEC and HITAEC have significant differences in the molecular profile of endothelial phenotype markers, basement membrane proteins, extracellular matrix components, and angiogenic proteins. © 2026, NII KPSSZ. All rights reserved.

KW - Basement membrane

KW - Coronary artery

KW - Endothelial cells

KW - Endothelial dysfunction

KW - Endothelial phenotype markers

KW - Extracellular matrix

KW - Internal thoracic artery

KW - aggrecanase 1

KW - alpha5 integrin

KW - angiogenic protein

KW - angiopoietin 2

KW - angiopoietin related protein 2

KW - beta3 integrin

KW - biglycan

KW - collagen type 4

KW - dipeptidyl peptidase I

KW - ephrin receptor B2

KW - ephrin receptor B4

KW - laminin

KW - molecular marker

KW - nrp1 protein

KW - nrp2 protein

KW - nultimerin 1

KW - ptprb protein

KW - scleroprotein

KW - tek protein

KW - transcription factor ERG

KW - transcription factor Fli 1

KW - unclassified drug

KW - vasculotropin receptor

KW - von Willebrand factor

KW - Article

KW - basement membrane

KW - controlled study

KW - coronary artery

KW - endoplasmic reticulum stress

KW - endothelial dysfunction

KW - extracellular matrix

KW - gene expression profiling

KW - gene overexpression

KW - high throughput sequencing

KW - human

KW - human cell

KW - internal mammary artery

KW - liquid chromatography-mass spectrometry

KW - primary cell

KW - protein expression

KW - RNA sequencing

KW - vascular endothelial cell

U2 - 10.17802/2306-1278-2025-14-6-80-104

DO - 10.17802/2306-1278-2025-14-6-80-104

M3 - статья

VL - 14

SP - 80

EP - 104

JO - Комплексные проблемы сердечно-сосудистых заболеваний

JF - Комплексные проблемы сердечно-сосудистых заболеваний

SN - 2306-1278

IS - 6

ER -

ID: 150125023