Frequencies of disk sequences in polytene chromosomes from sialadens of larvae of three chironomids spesies are analysed. Cases of a deflection of population frame from expected under Hardy-Vajnberg law are statistically confirmed. Allo-cation of frequencies of individuals with different level of heterozygosity in popu-lation of Camptochironomus tentans is investigated. Influence of driving and stabi-lising selection on population is shown. The mean of an assessment of influence of different disk sequences on fitness on the basis of single assemblies is offered. Sta-bility of nonequilibrium of the investigated populations as a homeostasis sign is noted.