Introduction. GIS is a modern computer technology designed for collecting, storing, analyzing and visualizing spatial data - mapping. GIS is also actively used in healthcare to solve many regional problems, including optimization and management of healthcare resources. Over last decade, many researchers have used mathematical software models for healthcare waste management. A review of the literature shows facility location, risk to the public, and economic feasibility to be among the issues that have been extensively covered in the literature. Designing a reverse logistics network using mathematical software tools is an efficient and effective way to manage healthcare waste. The objective of the study. To determine the optimal approach to creating a functional model of a system for managing medical waste of class B and C using GIS-technologies for each of the three regions, based on the characteristics of each. Materials and methods. Medical institutions in three regions (St. Petersburg, Leningrad Region and Krasnoyarsk Territory) as sources of medical waste generation were considered taking into account their capacity and specialization. The composition and volumes of waste by class were determined using a calculation method based on indicative waste generation standards. The identification of local technologies for the neutralization of medical waste existing in medical institutions was carried out. The obtained data was processed statistically and displayed on electronic maps for subsequent use in GIS technologies, as well as for the development and justification of a waste management system for medical institutions, taking into account the prospects for the development of healthcare. Results. Based on the analysis of data on medical waste of classes B and C in individual medical institutions, as well as an analysis of the power of existing technologies for neutralizing this waste using GIS technologies, proposals were developed to improve the medical waste management system in three regions. The proposals are aimed at improving the sanitary and epidemiological situation in the regions over a five-year period and take into account the features of the existing transport infrastructure and the requirements of sanitary legislation. Limitations. The model of a medical waste management system using GIS technologies was used only for managing waste of classes B and C. Conclusion. To ensure the safe and environmentally sustainable functioning of the waste management system of classes B and C in the regions, it is necessary to apply an integrated approach based on GIS technologies. This approach should take into account the regional characteristics of the management of the medical waste disposal system and its economic feasibility and take into account the regional characteristics of transport logistics and the prospects for the development of healthcare. © 2024 Federal Scientific Center of Hygiene named after FF Erisman. All rights reserved.