TY - JOUR

T1 - Биоинформатический анализ функционирования первичных артериальных эндотелиальных клеток в физиологическом состоянии на основании мультиомиксных данных.

AU - Маркова, Виктория

AU - Шишкова, Дарья

AU - Степанов, Александр

AU - Фролов, Алексей

AU - Изотова, Елизавета

AU - Юрьева, Юлия

AU - Лазебная , Анастасия

AU - Репкин, Егор Алексеевич

AU - Кабилов, Марсель

AU - Тупикин, Алексей

AU - Кутихин, Антон

PY - 2026/5/4

Y1 - 2026/5/4

N2 - Aim To perform an unbiased bioinformatic analysis of the molecular phenotype of intact endothelial cells derived from the atherosusceptible coronary artery (HCAEC) and the atheroresistant internal mammary artery (HITAEC). Methods Lysates of HCAEC and HITAEC were analyzed by whole transcriptome sequencing (RNA-seq) and ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Conditioned medium from HCAEC and HITAEC was also analyzed by UHPLC-MS/MS. Mean transcripts per million was used as the metric of gene expression, whilst protein expression was quantified based on chromatographic peak areas. Bioinformatic analysis of transcriptomic and proteomic data was performed using the Gene Ontology and Reactome databases. Results The most highly expressed endothelial phenotype markers in secretome and intracellular proteome of intact arterial ECs were vWF, EPCR/CD201, MCAM/ CD146, ICAM2/CD102, VE-cadherin/CDH5/CD144, and PECAM1/CD31. Notably, 30 out of 45 endothelial phenotype markers were released into the extracellular microenvironment even without pathological activation. Most endothelial cell adhesion molecules exhibited low basal expression and were inducible (ICAM1, NRCAM, ALCAM, SELE, VCAM1). Although arterial ECs expressed approximately 40 cytokine genes, only about 10 were synthesized and released at relatively significant levels (including MIF, PTX3, CSF1, CCL2, CCL14, IL-8/CXCL8, and CXCL1). ECs demonstrated high expression and secretion of almost all basement membrane components (approximately 20), as well as a substantial number of extracellular matrix components (approximately 65). Approximately 50 angiogenic and 65 hemostatic molecules were detected in the conditioned media of ECs. Higher number of transcribed genes and synthesized proteins involved in vasodilatory pathways compared to vasoconstrictive pathways indicated a more prominent role of intact ECs in vasodilation. Conclusion Intact ECs have high basal bioactivity in maintaining angiogenesis, hemostasis, and the synthesis of endothelial basement membrane and subendothelial extracellular matrix components. Albeit intact ECs exhibit low basal pro-inflammatory activity, they have a high potential for pathological activation in response to the respective stimuli.

AB - Aim To perform an unbiased bioinformatic analysis of the molecular phenotype of intact endothelial cells derived from the atherosusceptible coronary artery (HCAEC) and the atheroresistant internal mammary artery (HITAEC). Methods Lysates of HCAEC and HITAEC were analyzed by whole transcriptome sequencing (RNA-seq) and ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Conditioned medium from HCAEC and HITAEC was also analyzed by UHPLC-MS/MS. Mean transcripts per million was used as the metric of gene expression, whilst protein expression was quantified based on chromatographic peak areas. Bioinformatic analysis of transcriptomic and proteomic data was performed using the Gene Ontology and Reactome databases. Results The most highly expressed endothelial phenotype markers in secretome and intracellular proteome of intact arterial ECs were vWF, EPCR/CD201, MCAM/ CD146, ICAM2/CD102, VE-cadherin/CDH5/CD144, and PECAM1/CD31. Notably, 30 out of 45 endothelial phenotype markers were released into the extracellular microenvironment even without pathological activation. Most endothelial cell adhesion molecules exhibited low basal expression and were inducible (ICAM1, NRCAM, ALCAM, SELE, VCAM1). Although arterial ECs expressed approximately 40 cytokine genes, only about 10 were synthesized and released at relatively significant levels (including MIF, PTX3, CSF1, CCL2, CCL14, IL-8/CXCL8, and CXCL1). ECs demonstrated high expression and secretion of almost all basement membrane components (approximately 20), as well as a substantial number of extracellular matrix components (approximately 65). Approximately 50 angiogenic and 65 hemostatic molecules were detected in the conditioned media of ECs. Higher number of transcribed genes and synthesized proteins involved in vasodilatory pathways compared to vasoconstrictive pathways indicated a more prominent role of intact ECs in vasodilation. Conclusion Intact ECs have high basal bioactivity in maintaining angiogenesis, hemostasis, and the synthesis of endothelial basement membrane and subendothelial extracellular matrix components. Albeit intact ECs exhibit low basal pro-inflammatory activity, they have a high potential for pathological activation in response to the respective stimuli.

KW - Basement membrane

KW - Coronary artery

KW - Endothelial cells

KW - Endothelial phenotype markers

KW - Internal thoracic artery

KW - Physiological phenotype

KW - Pro-inflammatory cytokines

UR - https://www.mendeley.com/catalogue/4dfe7f1a-6f9b-31f6-8325-54fc48ff983c/

U2 - 10.17802/2306-1278-2026-15-2-187-205

DO - 10.17802/2306-1278-2026-15-2-187-205

M3 - статья

VL - 15

SP - 187

EP - 205

JO - Комплексные проблемы сердечно-сосудистых заболеваний

JF - Комплексные проблемы сердечно-сосудистых заболеваний

SN - 2306-1278

IS - 2

ER -