Mycological examination of tree and shrub vegetation on the territory of Saint Petersburg park zone and its suburbs was conducted. Material was collected in the parks of southern suburbs of Saint Petersburg (Pavlovsk Park, Catherine Park, Lower Garden and Upper Park in Oranienbaum, Upper Garden and Lower Park in Peterhof). For comparative purposes Park of Obukhov Hospital in Saint Petersburg city center was also examined. At the moment, 230 fungi species (micro- and macrofungi) were identified on trees and shrubs of the park zone. Among them, 28 species of microfungi, including powdery mildew, rust and blights pathogens were found on leaves. Also, 150 species of microfungi, including both common and rare fungi species, were found on branches and trunks. Most of them were found in the anamorphic stage. The greatest diversity and microfungi development were noted on dry branches. Thyrostromose of linden and Dutch elm disease are the most harmful. Stable complexes of pathogenic fungi, which development leads to clear decline of plants' condition, were identified. In addition, 52 species of macrofungi, including dominant and rare species, were observed on trunks of living and drying trees and vegetation residues. Among the sources of xylotrophic fungi infection of woody plants, dead elms are the most distinguished. Their dead trunks can be found in both suburban parks and the central part of Saint Petersburg. The presented data significantly expand available information on mycobiota Saint Petersburg parks.