The performed study of peculiarities of echo-detection of moving underwater targets by the Black Sea bottlenosed dolphins (Tursiops truncatus) suggests that the biosonar can purposefully change the structure of its probe signal, depending on the problem to be solved. It was shown experimentally for the first time that the animal emits the two- pulse probe signal to measure the constant velocity, while the three-pulse one, to measure acceleration. The provides an essential advantage over the single pulse, as it becomes possible to measure simultaneously parameters of the movement and distance.