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Whole genome sequencing data and analyses of the underlying SUP35 transcriptional regulation for a Saccharomyces cerevisiae nonsense suppressor mutant. / Matveenko, Andrew G.; Drozdova, Polina B.; Moskalenko, Svetlana E.; Tarasov, Oleg V.; Zhouravleva, Galina A.
в: Data in Brief, Том 23, 103694, 01.04.2019.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Whole genome sequencing data and analyses of the underlying SUP35 transcriptional regulation for a Saccharomyces cerevisiae nonsense suppressor mutant
AU - Matveenko, Andrew G.
AU - Drozdova, Polina B.
AU - Moskalenko, Svetlana E.
AU - Tarasov, Oleg V.
AU - Zhouravleva, Galina A.
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Termination of translation in eukaryotes is governed by two release factors encoded by the SUP45 and SUP35 genes in Saccharomyces cerevisiae. Previously, a set of mutations in these genes had been obtained. However, the exact sequence change associated with one mutation, sup35-222, was not identified by Sanger sequencing of the SUP35 region. Presented here are whole-genome sequencing data for the sup35-222 strain, data on copy number variation in its genome along with supporting pulse-field gel electrophoresis experiment data, and the list of single-nucleotide variations that differentiate this strain and its wild-type ancestor. One substitution upstream the SUP35 gene was located in a sequence corresponding to the Abf1-binding site. Data obtained from the introduction of this variation from sup35-222 strain into a different wild-type strain, specifically, detection of a nonsense-suppressor phenotype accompanied by a decrease in the Sup35 protein level, are also presented in this article.
AB - Termination of translation in eukaryotes is governed by two release factors encoded by the SUP45 and SUP35 genes in Saccharomyces cerevisiae. Previously, a set of mutations in these genes had been obtained. However, the exact sequence change associated with one mutation, sup35-222, was not identified by Sanger sequencing of the SUP35 region. Presented here are whole-genome sequencing data for the sup35-222 strain, data on copy number variation in its genome along with supporting pulse-field gel electrophoresis experiment data, and the list of single-nucleotide variations that differentiate this strain and its wild-type ancestor. One substitution upstream the SUP35 gene was located in a sequence corresponding to the Abf1-binding site. Data obtained from the introduction of this variation from sup35-222 strain into a different wild-type strain, specifically, detection of a nonsense-suppressor phenotype accompanied by a decrease in the Sup35 protein level, are also presented in this article.
KW - YEAST
KW - MUTATIONS
KW - STABILITY
KW - FORMAT
KW - SUP45
UR - http://www.scopus.com/inward/record.url?scp=85061189850&partnerID=8YFLogxK
U2 - 10.1016/j.dib.2019.01.042
DO - 10.1016/j.dib.2019.01.042
M3 - Article
AN - SCOPUS:85061189850
VL - 23
JO - Data in Brief
JF - Data in Brief
SN - 2352-3409
M1 - 103694
ER -
ID: 47423356