TY - JOUR

T1 - Vliianie lokal'nogo UF mikrooblucheniia na iadernyǐ apparat i tsitoplazmu infuzoriǐ Paramecium caudatum

AU - Fokin, S. I.

AU - Osipov, D. V.

PY - 1975/9/1

Y1 - 1975/9/1

N2 - The work is the first attempt to study nuclear dualism of ciliates with ultraviolet microbeam (UV-beam), which was not applied earlier for these purposes. The special advantage of the UV-beam is that it allow to inactivate selectively of the particular elements of nuclear apparatus of living ciliates is to observe consequences of operation on distant descendants of irradiated cell. On Paramecium caudatum the ways of UV-beam have been devised specifically. The original rotocompression chamber was employed for immobilization of cell in space of irradiation. The effective UV-doses were selected, the relative resistance of cytoplasm, macro-, micronucleus (Mi), and symbiotic bacteria of Mi -- omega particles -- was detected. The "pure" and infected cells were irradiated, in some different variants. The effect of UV-beam depends on dose and consists in a reduction the effectiveness of cell cloning, the loss of omega-particles from Mi, the appearance of the cells with 2--3 Mi, and, possibly, of amicronucleate cells. The injuries due to UV-beam are restored by photoreactivation of visible light.

AB - The work is the first attempt to study nuclear dualism of ciliates with ultraviolet microbeam (UV-beam), which was not applied earlier for these purposes. The special advantage of the UV-beam is that it allow to inactivate selectively of the particular elements of nuclear apparatus of living ciliates is to observe consequences of operation on distant descendants of irradiated cell. On Paramecium caudatum the ways of UV-beam have been devised specifically. The original rotocompression chamber was employed for immobilization of cell in space of irradiation. The effective UV-doses were selected, the relative resistance of cytoplasm, macro-, micronucleus (Mi), and symbiotic bacteria of Mi -- omega particles -- was detected. The "pure" and infected cells were irradiated, in some different variants. The effect of UV-beam depends on dose and consists in a reduction the effectiveness of cell cloning, the loss of omega-particles from Mi, the appearance of the cells with 2--3 Mi, and, possibly, of amicronucleate cells. The injuries due to UV-beam are restored by photoreactivation of visible light.

UR - http://www.scopus.com/inward/record.url?scp=0016551173&partnerID=8YFLogxK

M3 - статья

C2 - 1166524

AN - SCOPUS:0016551173

VL - 17

SP - 1073

EP - 1080

JO - Цитология

JF - Цитология

SN - 0041-3771

IS - 9

ER -