Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Vesicle Shrinking and Enlargement Play Opposing Roles in the Release of Exocytotic Contents. / Shin, Wonchul; Arpino, Gianvito; Thiyagarajan, Sathish; Su, Rui; Ge, Lihao; McDargh, Zachary ; Guo, Xiaoli; Wei, Lisi; Shupliakov, Oleg V.; Jin, Albert; O'Shaughnessy, Ben; Wu, Ling Gang.
в: Cell Reports, Том 30, № 2, 14.01.2020, стр. 421-431.e7.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Vesicle Shrinking and Enlargement Play Opposing Roles in the Release of Exocytotic Contents
AU - Shin, Wonchul
AU - Arpino, Gianvito
AU - Thiyagarajan, Sathish
AU - Su, Rui
AU - Ge, Lihao
AU - McDargh, Zachary
AU - Guo, Xiaoli
AU - Wei, Lisi
AU - Shupliakov, Oleg V.
AU - Jin, Albert
AU - O'Shaughnessy, Ben
AU - Wu, Ling Gang
N1 - Publisher Copyright: © 2019
PY - 2020/1/14
Y1 - 2020/1/14
N2 - For decades, two fusion modes were thought to control hormone and transmitter release essential to life; one facilitates release via fusion pore dilation and flattening (full collapse), and the other limits release by closing a narrow fusion pore (kiss-and-run). Using super-resolution stimulated emission depletion (STED) microscopy to visualize fusion modes of dense-core vesicles in neuroendocrine cells, we find that facilitation of release is mediated not by full collapse but by shrink fusion, in which the Ω-profile generated by vesicle fusion shrinks but maintains a large non-dilating pore. We discover that the physiological osmotic pressure of a cell squeezes, but does not dilate, the Ω-profile, which explains why shrink fusion prevails over full collapse. Instead of kiss-and-run, enlarge fusion, in which Ω-profiles grow while maintaining a narrow pore, slows down release. Shrink and enlarge fusion may thus account for diverse hormone and transmitter release kinetics observed in secretory cells, previously interpreted within the full-collapse/kiss-and-run framework.
AB - For decades, two fusion modes were thought to control hormone and transmitter release essential to life; one facilitates release via fusion pore dilation and flattening (full collapse), and the other limits release by closing a narrow fusion pore (kiss-and-run). Using super-resolution stimulated emission depletion (STED) microscopy to visualize fusion modes of dense-core vesicles in neuroendocrine cells, we find that facilitation of release is mediated not by full collapse but by shrink fusion, in which the Ω-profile generated by vesicle fusion shrinks but maintains a large non-dilating pore. We discover that the physiological osmotic pressure of a cell squeezes, but does not dilate, the Ω-profile, which explains why shrink fusion prevails over full collapse. Instead of kiss-and-run, enlarge fusion, in which Ω-profiles grow while maintaining a narrow pore, slows down release. Shrink and enlarge fusion may thus account for diverse hormone and transmitter release kinetics observed in secretory cells, previously interpreted within the full-collapse/kiss-and-run framework.
KW - bovine chromaffin cells
KW - exocytosis
KW - hormone release
KW - mathematical modeling
KW - membrane dynamics
KW - membrane fusion
KW - osmotic pressure
KW - transmitter release
KW - vesicle enlargement
KW - vesicle shrinking
UR - https://www.ncbi.nlm.nih.gov/pubmed/31940486
UR - http://www.scopus.com/inward/record.url?scp=85077934619&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2019.12.044
DO - 10.1016/j.celrep.2019.12.044
M3 - Article
VL - 30
SP - 421-431.e7
JO - Cell Reports
JF - Cell Reports
SN - 2639-1856
IS - 2
ER -
ID: 52334436