TY - JOUR

T1 - Vannella samoroda n. sp. (Amoebozoa) – first member of the genus from a continental saline habitat placed in a molecular tree

AU - Kudryavtsev, Alexander

AU - Volkova, Ekaterina

AU - Plotnikov, Andrey

N1 - Publisher Copyright: © 2019 Elsevier GmbH

PY - 2019/10

Y1 - 2019/10

N2 - Vannella samoroda n. sp. (Amoebozoa, Vannellida) was isolated from the mouth of the Malaya Samoroda river flowing into Elton, the largest European hypersaline lake (Russia). Among all rivers of the area, it has the highest salt content (ca. 110‰). Amoebae maintained in seawater medium with ca. 77‰ salts concentration had a set of morphological characters typical of Vannella spp.: rounded, fan-shaped, or spatulate locomotive form, floating form with bent, blunt-ended hyaline pseudopodia, and a cell coat consisting of regularly packed palisade elements and scarce simple filaments. Phylogenetic analyses based on SSU rRNA and cytochrome C oxidase subunit 1 genes show that the amoeba is most closely related to Vannella ebro Smirnov, 2001, but represents a distinct species. The clade of V. ebro and V. samoroda branches among marine species of Vannella. The studied species is the first member of the genus Vannella from a continental saline habitat described using molecular data. Interestingly, it has a broad range of salinity tolerance: cells reproduce above 18‰, while survival of a few cells regularly occurs even in highly diluted Prescott and James medium. The normal culture restores itself when PJ medium is substituted with 77‰ seawater medium even after months of experimental incubation.

AB - Vannella samoroda n. sp. (Amoebozoa, Vannellida) was isolated from the mouth of the Malaya Samoroda river flowing into Elton, the largest European hypersaline lake (Russia). Among all rivers of the area, it has the highest salt content (ca. 110‰). Amoebae maintained in seawater medium with ca. 77‰ salts concentration had a set of morphological characters typical of Vannella spp.: rounded, fan-shaped, or spatulate locomotive form, floating form with bent, blunt-ended hyaline pseudopodia, and a cell coat consisting of regularly packed palisade elements and scarce simple filaments. Phylogenetic analyses based on SSU rRNA and cytochrome C oxidase subunit 1 genes show that the amoeba is most closely related to Vannella ebro Smirnov, 2001, but represents a distinct species. The clade of V. ebro and V. samoroda branches among marine species of Vannella. The studied species is the first member of the genus Vannella from a continental saline habitat described using molecular data. Interestingly, it has a broad range of salinity tolerance: cells reproduce above 18‰, while survival of a few cells regularly occurs even in highly diluted Prescott and James medium. The normal culture restores itself when PJ medium is substituted with 77‰ seawater medium even after months of experimental incubation.

KW - Hypersaline biotopes

KW - Molecular phylogeny

KW - Morphology

KW - New combination

KW - Salinity tolerance

KW - Vannella

KW - DACTYLOPODIDA

KW - PHYLOGENETIC ANALYSIS

KW - SUBUNIT RIBOSOMAL-RNA

KW - LOBOSEA

KW - TAXONOMY

KW - AMEBAS

KW - SALTON-SEA

KW - PROTOZOA

KW - DISCOSEA

KW - GYMNAMOEBIA

UR - http://www.scopus.com/inward/record.url?scp=85072699979&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/vannella-samoroda-n-sp-amoebozoa-first-member-genus-continental-saline-habitat-placed-molecular-tre

U2 - 10.1016/j.ejop.2019.125634

DO - 10.1016/j.ejop.2019.125634

M3 - Article

VL - 71

JO - European Journal of Protistology

JF - European Journal of Protistology

SN - 0932-4739

M1 - 125634

ER -