Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Vannella samoroda n. sp. (Amoebozoa) – first member of the genus from a continental saline habitat placed in a molecular tree. / Kudryavtsev, Alexander ; Volkova, Ekaterina ; Plotnikov, Andrey .
в: European Journal of Protistology, Том 71, 125634, 10.2019.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Vannella samoroda n. sp. (Amoebozoa) – first member of the genus from a continental saline habitat placed in a molecular tree
AU - Kudryavtsev, Alexander
AU - Volkova, Ekaterina
AU - Plotnikov, Andrey
N1 - Publisher Copyright: © 2019 Elsevier GmbH
PY - 2019/10
Y1 - 2019/10
N2 - Vannella samoroda n. sp. (Amoebozoa, Vannellida) was isolated from the mouth of the Malaya Samoroda river flowing into Elton, the largest European hypersaline lake (Russia). Among all rivers of the area, it has the highest salt content (ca. 110‰). Amoebae maintained in seawater medium with ca. 77‰ salts concentration had a set of morphological characters typical of Vannella spp.: rounded, fan-shaped, or spatulate locomotive form, floating form with bent, blunt-ended hyaline pseudopodia, and a cell coat consisting of regularly packed palisade elements and scarce simple filaments. Phylogenetic analyses based on SSU rRNA and cytochrome C oxidase subunit 1 genes show that the amoeba is most closely related to Vannella ebro Smirnov, 2001, but represents a distinct species. The clade of V. ebro and V. samoroda branches among marine species of Vannella. The studied species is the first member of the genus Vannella from a continental saline habitat described using molecular data. Interestingly, it has a broad range of salinity tolerance: cells reproduce above 18‰, while survival of a few cells regularly occurs even in highly diluted Prescott and James medium. The normal culture restores itself when PJ medium is substituted with 77‰ seawater medium even after months of experimental incubation.
AB - Vannella samoroda n. sp. (Amoebozoa, Vannellida) was isolated from the mouth of the Malaya Samoroda river flowing into Elton, the largest European hypersaline lake (Russia). Among all rivers of the area, it has the highest salt content (ca. 110‰). Amoebae maintained in seawater medium with ca. 77‰ salts concentration had a set of morphological characters typical of Vannella spp.: rounded, fan-shaped, or spatulate locomotive form, floating form with bent, blunt-ended hyaline pseudopodia, and a cell coat consisting of regularly packed palisade elements and scarce simple filaments. Phylogenetic analyses based on SSU rRNA and cytochrome C oxidase subunit 1 genes show that the amoeba is most closely related to Vannella ebro Smirnov, 2001, but represents a distinct species. The clade of V. ebro and V. samoroda branches among marine species of Vannella. The studied species is the first member of the genus Vannella from a continental saline habitat described using molecular data. Interestingly, it has a broad range of salinity tolerance: cells reproduce above 18‰, while survival of a few cells regularly occurs even in highly diluted Prescott and James medium. The normal culture restores itself when PJ medium is substituted with 77‰ seawater medium even after months of experimental incubation.
KW - Hypersaline biotopes
KW - Molecular phylogeny
KW - Morphology
KW - New combination
KW - Salinity tolerance
KW - Vannella
KW - DACTYLOPODIDA
KW - PHYLOGENETIC ANALYSIS
KW - SUBUNIT RIBOSOMAL-RNA
KW - LOBOSEA
KW - TAXONOMY
KW - AMEBAS
KW - SALTON-SEA
KW - PROTOZOA
KW - DISCOSEA
KW - GYMNAMOEBIA
UR - http://www.scopus.com/inward/record.url?scp=85072699979&partnerID=8YFLogxK
UR - http://www.mendeley.com/research/vannella-samoroda-n-sp-amoebozoa-first-member-genus-continental-saline-habitat-placed-molecular-tre
U2 - 10.1016/j.ejop.2019.125634
DO - 10.1016/j.ejop.2019.125634
M3 - Article
VL - 71
JO - European Journal of Protistology
JF - European Journal of Protistology
SN - 0932-4739
M1 - 125634
ER -
ID: 45258944