TY - JOUR

T1 - Truncated hemoglobin 2 modulates phosphorus deficiency response by controlling of gene expression in nitric oxide-dependent pathway in Chlamydomonas reinhardtii

AU - Grinko, Alexandra

AU - Alqoubaili, Reem

AU - Lapina, Tatiana

AU - Ermilova, Elena

N1 - Publisher Copyright: © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2021/7/28

Y1 - 2021/7/28

N2 - Main conclusion: Truncated hemoglobin 2 is involved in fine-tuning of PSR1-regulated gene expression during phosphorus deprivation. Abstract: Truncated hemoglobins form a large family found in all domains of life. However, a majority of physiological functions of these proteins remain to be elucidated. In the model alga Chlamydomonas reinhardtii, macro-nutritional deprivation is known to elevate truncated hemoglobin 2 (THB2). This study investigated the role of THB2 in the regulation of a subset of phosphorus (P) limitation-responsive genes in cells suffering from P-deficiency. Underexpression of THB2 in amiTHB2 strains resulted in downregulation of a suite of P deprivation-induced genes encoding proteins with different subcellular location and functions (e.g., PHOX, LHCSR3.1, LHCSR3.2, PTB2, and PTB5). Moreover, our results provided primary evidence that the soluble guanylate cyclase 12 gene (CYG12) is a component of the P deprivation regulation. Furthermore, the transcription of PSR1 gene for the most critical regulator in the acclimation process under P restriction was repressed by nitric oxide (NO). Collectively, the results indicated a tight regulatory link between the THB2-controlled NO levels and PSR1-dependent induction of several P deprivation responsive genes with various roles in cells during P-limitation.

AB - Main conclusion: Truncated hemoglobin 2 is involved in fine-tuning of PSR1-regulated gene expression during phosphorus deprivation. Abstract: Truncated hemoglobins form a large family found in all domains of life. However, a majority of physiological functions of these proteins remain to be elucidated. In the model alga Chlamydomonas reinhardtii, macro-nutritional deprivation is known to elevate truncated hemoglobin 2 (THB2). This study investigated the role of THB2 in the regulation of a subset of phosphorus (P) limitation-responsive genes in cells suffering from P-deficiency. Underexpression of THB2 in amiTHB2 strains resulted in downregulation of a suite of P deprivation-induced genes encoding proteins with different subcellular location and functions (e.g., PHOX, LHCSR3.1, LHCSR3.2, PTB2, and PTB5). Moreover, our results provided primary evidence that the soluble guanylate cyclase 12 gene (CYG12) is a component of the P deprivation regulation. Furthermore, the transcription of PSR1 gene for the most critical regulator in the acclimation process under P restriction was repressed by nitric oxide (NO). Collectively, the results indicated a tight regulatory link between the THB2-controlled NO levels and PSR1-dependent induction of several P deprivation responsive genes with various roles in cells during P-limitation.

KW - Acclimation responses

KW - Green algae

KW - Nutrient scavenging

KW - PSR1

KW - Soluble guanylate cyclase 12

KW - PROTEIN

KW - CHLOROPLAST

KW - MECHANISMS

KW - PHOSPHATE

KW - METABOLISM

KW - DEPRIVATION

KW - ACCLIMATION

KW - PLANTS

KW - NITRATE REDUCTASE

KW - STRESS

UR - http://www.scopus.com/inward/record.url?scp=85111377543&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/d1ee9dd4-6e09-3a9d-8c16-9beb5d425131/

U2 - 10.1007/s00425-021-03691-4

DO - 10.1007/s00425-021-03691-4

M3 - Article

AN - SCOPUS:85111377543

VL - 254

JO - Planta

JF - Planta

SN - 0032-0935

IS - 2

M1 - 39

ER -